Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Long-Ho Chau
  • Wenfeng Liang
  • Florence Wing Ki Cheung
  • Wing Keung Liu
  • Shih-Chi Chen
  • Gwo-Bin Lee

Detail(s)

Original languageEnglish
Article numbere51577
Journal / PublicationPLoS ONE
Volume8
Issue number1
Online published8 Jan 2013
Publication statusPublished - Jan 2013

Link(s)

Abstract

The use of optical dielectrophoresis (ODEP) to manipulate microparticles and biological cells has become increasingly popular due to its tremendous flexibility in providing reconfigurable electrode patterns and flow channels. ODEP enables the parallel and free manipulation of small particles on a photoconductive surface on which light is projected, thus eliminating the need for complex electrode design and fabrication processes. In this paper, we demonstrate that mouse cells comprising melan-a cells, RAW 267.4 macrophage cells, peripheral white blood cells and lymphocytes, can be manipulated in an opto-electrokinetics (OEK) device with appropriate DEP parameters. Our OEK device generates a non-rotating electric field and exerts a localized DEP force on optical electrodes. Hitherto, we are the first group to report that among all the cells investigated, melan-a cells, lymphocytes and white blood cells were found to undergo self-rotation in the device in the presence of a DEP force. The rotational speed of the cells depended on the voltage and frequency applied and the cells' distance from the optical center. We discuss a possible mechanism for explaining this new observation of induced self-rotation based on the physical properties of cells. We believe that this rotation phenomenon can be used to identify cell type and to elucidate the dielectric and physical properties of cells.

Research Area(s)

Citation Format(s)

Self-Rotation of Cells in an Irrotational AC E-Field in an Opto-Electrokinetics Chip. / Chau, Long-Ho; Liang, Wenfeng; Cheung, Florence Wing Ki; Liu, Wing Keung; Li, Wen Jung; Chen, Shih-Chi; Lee, Gwo-Bin.

In: PLoS ONE, Vol. 8, No. 1, e51577, 01.2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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