Self-aligned supported lipid bilayers for patterning the cell-substrate interface

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Keyue Shen
  • Jones Tsai
  • Peng Shi
  • Lance C. Kam

Detail(s)

Original languageEnglish
Pages (from-to)13204-13205
Journal / PublicationJournal of the American Chemical Society
Volume131
Issue number37
Publication statusPublished - 23 Sep 2009
Externally publishedYes

Abstract

(Figure Presented) Supported lipid bilayers capture the fluidity and chemical properties of cellular membranes. In this report, we introduce a method for creating surfaces that contain multiple, aligned regions of supported membranes of different compositions at scales of micrometers and smaller. This method uses the design of a diffusional barrier to increase the resolution that can be achieved directly using traditional bilayer patterning techniques, such as laminar flow. We demonstrate the use of this platform for presenting ligands to the T Cell Receptor and LFA-1 that are tethered to separate, closely juxtaposed regions of bilayer, capturing an important aspect of the natural organization observed between T cells and Antigen Presenting Cells. Our results present a novel platform for the study of spatial separation of extracellular ligands and its impact on cell signals. © 2009 American Chemical Society.

Citation Format(s)

Self-aligned supported lipid bilayers for patterning the cell-substrate interface. / Shen, Keyue; Tsai, Jones; Shi, Peng; Kam, Lance C.

In: Journal of the American Chemical Society, Vol. 131, No. 37, 23.09.2009, p. 13204-13205.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review