Screening for methicillin-resistant staphylococci in dogs admitted to a veterinary teaching hospital

Wannes Vanderhaeghen, Evelien Van De Velde, Florence Crombé, Ingeborgh Polis, Katleen Hermans, Freddy Haesebrouck, Patrick Butaye

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

11 Citations (Scopus)

Abstract

This study investigated the nasal carriage of methicillin-resistant staphylococci (MRS) in dogs (n= 177) prior to medical examination or surgery in a veterinary teaching hospital. Nasal swab samples were collected after induction of anaesthesia and incubated overnight in salt enriched trypticase-soy broth. Cultures were analysed on two different agar media containing cefoxitin. Suspected MRS isolates were genotypically identified and characterised by antimicrobial susceptibility testing and staphylococcal cassette chromosome mec (SCC. mec)-typing. Methicillin-resistant Staphylococcus aureus (MRSA) isolates were additionally characterised by spa-typing and multilocus sequence typing. The presence of Panton-Valentine leukocidin (pvl) genes was determined by PCR. MRS carriage was compared between animals with or without an infectious process. Two MRSA were isolated, both belonging to typical Belgian human hospital clones and lacking pvl. Additionally a methicillin-resistant Staphylococcus haemolyticus carrying a type V SCC. mec was detected. No relationship was observed between MRS carriage and presence of infections.The results suggest that MRS are present in dogs originating from the community, albeit at a low prevalence. This could pose risks for cross contamination of dogs and their owners. © 2011 Elsevier Ltd.
Original languageEnglish
Pages (from-to)133-136
JournalResearch in Veterinary Science
Volume93
Issue number1
DOIs
Publication statusPublished - Aug 2012
Externally publishedYes

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Research Keywords

  • Carriage
  • Coagulase-negative staphylococci
  • Dogs
  • Methicillin-resistant staphylococci
  • MRSA

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