Sandwich-structured upconversion nanoparticles with tunable color for multiplexed cell labeling

Qingqing Dou; Niagara Muhammad Idris; Yong Zhang

doi:10.1016/j.biomaterials.2012.11.011

Sandwich-structured upconversion nanoparticles with tunable color for multiplexed cell labeling

Qingqing Dou
, Niagara Muhammad Idris
, Yong Zhang

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

116 Citations (Scopus)

Abstract

The need for a more efficient biological label to meet their burgeoning utility in rapidly developing multiplexing applications may be realized through the recent advent of upconversion nanoparticles (UCNs). UCNs fabricated to-date, however, are either not displaying strong fluorescence or have limited available colors. Here, we report on fabricating sandwich-structured UCNs with a NaYbF₄ matrix sandwiched between two NaYF₄ layers. Such sandwich design allows for efficient absorption of the excitation energy by the absorber ion-rich NaYbF₄ layer that then transfers it to the adjacent NaYF₄ layers on either side for an improved fluorescence efficiency. By doping different emitters into each of the shells and adjusting their thickness, different color output tunable based on the RGB color model were obtained. In this study, multicolor UCNs with strong emission intensity have been facilely synthesized and used for multiplex detection of three subcellular targets with a single near-infrared excitation wavelength. © 2012 Elsevier Ltd.

Original language	English
Pages (from-to)	1722-1731
Journal	Biomaterials
Volume	34
Issue number	6
DOIs	https://doi.org/10.1016/j.biomaterials.2012.11.011
Publication status	Published - Feb 2013
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Funding

Financial support from A*STAR BMRC (R-397-000-119-305) and National University of Singapore are gratefully acknowledged.

Research Keywords

Cell culture
Confocal microscopy
Fluorescence
Nanoparticle

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title = "Sandwich-structured upconversion nanoparticles with tunable color for multiplexed cell labeling",

abstract = "The need for a more efficient biological label to meet their burgeoning utility in rapidly developing multiplexing applications may be realized through the recent advent of upconversion nanoparticles (UCNs). UCNs fabricated to-date, however, are either not displaying strong fluorescence or have limited available colors. Here, we report on fabricating sandwich-structured UCNs with a NaYbF4 matrix sandwiched between two NaYF4 layers. Such sandwich design allows for efficient absorption of the excitation energy by the absorber ion-rich NaYbF4 layer that then transfers it to the adjacent NaYF4 layers on either side for an improved fluorescence efficiency. By doping different emitters into each of the shells and adjusting their thickness, different color output tunable based on the RGB color model were obtained. In this study, multicolor UCNs with strong emission intensity have been facilely synthesized and used for multiplex detection of three subcellular targets with a single near-infrared excitation wavelength. {\textcopyright} 2012 Elsevier Ltd.",

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AU - Idris, Niagara Muhammad

AU - Zhang, Yong

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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N2 - The need for a more efficient biological label to meet their burgeoning utility in rapidly developing multiplexing applications may be realized through the recent advent of upconversion nanoparticles (UCNs). UCNs fabricated to-date, however, are either not displaying strong fluorescence or have limited available colors. Here, we report on fabricating sandwich-structured UCNs with a NaYbF4 matrix sandwiched between two NaYF4 layers. Such sandwich design allows for efficient absorption of the excitation energy by the absorber ion-rich NaYbF4 layer that then transfers it to the adjacent NaYF4 layers on either side for an improved fluorescence efficiency. By doping different emitters into each of the shells and adjusting their thickness, different color output tunable based on the RGB color model were obtained. In this study, multicolor UCNs with strong emission intensity have been facilely synthesized and used for multiplex detection of three subcellular targets with a single near-infrared excitation wavelength. © 2012 Elsevier Ltd.

AB - The need for a more efficient biological label to meet their burgeoning utility in rapidly developing multiplexing applications may be realized through the recent advent of upconversion nanoparticles (UCNs). UCNs fabricated to-date, however, are either not displaying strong fluorescence or have limited available colors. Here, we report on fabricating sandwich-structured UCNs with a NaYbF4 matrix sandwiched between two NaYF4 layers. Such sandwich design allows for efficient absorption of the excitation energy by the absorber ion-rich NaYbF4 layer that then transfers it to the adjacent NaYF4 layers on either side for an improved fluorescence efficiency. By doping different emitters into each of the shells and adjusting their thickness, different color output tunable based on the RGB color model were obtained. In this study, multicolor UCNs with strong emission intensity have been facilely synthesized and used for multiplex detection of three subcellular targets with a single near-infrared excitation wavelength. © 2012 Elsevier Ltd.

KW - Cell culture

KW - Confocal microscopy

KW - Fluorescence

KW - Nanoparticle

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U2 - 10.1016/j.biomaterials.2012.11.011

DO - 10.1016/j.biomaterials.2012.11.011

M3 - RGC 21 - Publication in refereed journal

C2 - 23201249

SN - 0142-9612

VL - 34

SP - 1722

EP - 1731

JO - Biomaterials

JF - Biomaterials

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ER -

Sandwich-structured upconversion nanoparticles with tunable color for multiplexed cell labeling

Abstract

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Research Keywords

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