R-stereopreference analysis of lipase Novozym®435 in kinetic resolution of flurbiprofen

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

11 Scopus Citations
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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)245-249
Journal / PublicationChirality
Volume19
Issue number4
Publication statusPublished - 2007
Externally publishedYes

Abstract

Immobilized lipase from Candida antarctica (Novozym®435) was employed in the kinetic resolution of racemic flurbiprofen by enantioselective esterification with methanol. It was found that the lipase has the R-stereopreference and the reaction matches Bi Bi Ping Pong mechanism with dead-end inhibition of methanol. Furthermore, the R-stereopreference was analyzed in details from the aspects of enzymatic kinetic mechanism and reaction activation energy of both enantiomers. The R-enantiomer shows lower activation energy and higher maximum reaction rate than the S-enantiomer, which implies the R-stereopreference of the lipase and makes the kinetic resolution of flurbiprofen via enzymatic reaction feasible. © 2006 Wiley-Liss, Inc.

Research Area(s)

  • Activation energy, Flurbiprofen, Maximum reaction rate, Novozym®435, Stereopreference

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Citation Format(s)

R-stereopreference analysis of lipase Novozym®435 in kinetic resolution of flurbiprofen. / Hua, Yun Zhang; Wang, Xin; Chi, Bun Ching.
In: Chirality, Vol. 19, No. 4, 2007, p. 245-249.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review