Roles of nitric oxide in adaptive response induced in zebrafish embryos in vivo by microbeam protons

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

View graph of relations

Author(s)

  • Viann Wing Yan Choi
  • Candy Yuen Ping NG
  • Alisa KOBAYASHI
  • Teruaki KONISHI
  • Masakazu OIKAWA

Detail(s)

Original languageEnglish
Pages (from-to)i114
Journal / PublicationJournal of Radiation Research
Volume55
Issue numbersuppl_1
Online published1 Mar 2014
Publication statusPublished - Mar 2014

Conference

TitleHeavy Ion in Therapy and Space Radiation Symposium 2013 (HITSRS2013)
LocationKelyo Bank Culture Plaza
PlaceJapan
CityChiba
Period15 - 18 May 2013

Link(s)

Abstract

Radioadaptive response (RAR) was successfully induced in dechorionated (5 h post-fertilization, hpf) embryos of the zebrafish, Danio rerio, by 3.4 MeV protons from the microbeam irradiation facility (Single-Particle Irradiation System to Cell, acronym as SPICE) [1] at the National Institute of Radiological Sciences (NIRS), against a challenging exposure of 2 Gy of X-ray irradiation at 10 hpf. The RAR induction was corroborated by reduced apoptotic signals at 25 hpf revealed through terminal dUTP transferase-mediated nick end-labeling assay. If de novo synthesis of factors was required for RAR induction, these should have already been synthesized at 5 h after the priming dose.

Application of a nitric oxide scavenger 2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) to the medium at 0, 1, 2, 3 or 5 h after application of priming exposure significantly suppressed RAR. The suppression of RAR with the application of cPTIO to the medium at 5 h after the priming dose irradiation, where de novo synthesis of factors should have been completed, suggested that NO scavenging impaired the repair machineries in the bystander cells. The suppression of RAR with the application of cPTIO to the medium at earlier than 5 h after the priming dose irradiation could be explained by the scavenging of bystander NO signals in the medium and thus deterring the de novo synthesis of factors.

Research Area(s)

Citation Format(s)

Roles of nitric oxide in adaptive response induced in zebrafish embryos in vivo by microbeam protons. / Choi, Viann Wing Yan; NG, Candy Yuen Ping; KOBAYASHI, Alisa; KONISHI, Teruaki; OIKAWA, Masakazu; CHENG, Shuk Han; YU, Kwan Ngok.

In: Journal of Radiation Research, Vol. 55, No. suppl_1, 03.2014, p. i114.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review