Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1

Bora Jeong; Han Rae Kim; Nan Seong Choi; Byong Seo Park; Hyejin Eom; Jeong Woo Park; Jae Geun Kim; Byung Ju Lee

doi:10.1016/j.bbrc.2018.01.014

Author(s)

Bora Jeong
Han Rae Kim
Nan Seong Choi
Byong Seo Park
Hyejin Eom
And 3 others

Jeong Woo Park
Jae Geun Kim
Byung Ju Lee

Related Research Unit(s)

Department of Biomedical Sciences

Detail(s)

Original language	English
Pages (from-to)	147-152
Journal / Publication	Biochemical and Biophysical Research Communications
Volume	496
Issue number	1
Online published	4 Jan 2018
Publication status	Published - 29 Jan 2018

Link(s)

DOI	DOI https://doi.org/10.1016/j.bbrc.2018.01.014 Final Published version
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Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85042077479&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(7787f36d-7f14-47be-a633-767e3655c190).html

Abstract

Here, we report thyroid transcription factor 1 (TTF-1) as an important transcription factor for the expression of heme oxygenase-1 (HO-1). HO-1 is a well-known cytoprotective enzyme against inflammation. We observed that HO-1 co-expressed with TTF-1 in mouse hypothalamic cells. Results from luciferase and chromatin immunoprecipitation assays revealed that TTF-1 directly activated HO-1 transcription by binding to binding domains in the 5'-flanking region of the HO-1 gene. A proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α), induced nuclear translocation of TTF-1 and increased binding affinity of TTF-1 to its binding sites on the HO-1 gene. HO-1 mRNA increased with TTF-1 overexpression but decreased with RNA interference of TTF-1 expression in rat astroglial C6 cells. Together with results showing involvement of TTF-1 in the TNF-α-induced increase in interleukin 1 beta and monocyte chemotactic protein 1 production, this study suggests that TTF-1 plays an important role in the mouse hypothalamus TNF-α-induced inflammatory response for regulating HO-1 gene expression.

Research Area(s)

Astroglial activation, Heme oxygenase-1, Hypothalamic inflammation, Thyroid transcription factor-1

SDG 3 - Good Health and Well-being

Citation Format(s)

[ RIS ] [ BibTeX ]

Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1. / Jeong, Bora; Kim, Han Rae; Choi, Nan Seong et al.
In: Biochemical and Biophysical Research Communications, Vol. 496, No. 1, 29.01.2018, p. 147-152.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Jeong, B, Kim, HR, Choi, NS, Park, BS, Eom, H, Park, JW, Kim, JG & Lee, BJ 2018, 'Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1', Biochemical and Biophysical Research Communications, vol. 496, no. 1, pp. 147-152. https://doi.org/10.1016/j.bbrc.2018.01.014

Jeong, B., Kim, H. R., Choi, N. S., Park, B. S., Eom, H., Park, J. W., Kim, J. G., & Lee, B. J. (2018). Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1. Biochemical and Biophysical Research Communications, 496(1), 147-152. https://doi.org/10.1016/j.bbrc.2018.01.014

Jeong B, Kim HR, Choi NS, Park BS, Eom H, Park JW et al. Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1. Biochemical and Biophysical Research Communications. 2018 Jan 29;496(1):147-152. Epub 2018 Jan 4. doi: 10.1016/j.bbrc.2018.01.014

Jeong, Bora ; Kim, Han Rae ; Choi, Nan Seong et al. / Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 496, No. 1. pp. 147-152.