Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
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Author(s)

  • Bora Jeong
  • Han Rae Kim
  • Nan Seong Choi
  • Byong Seo Park
  • Hyejin Eom
  • And 3 others
  • Jeong Woo Park
  • Jae Geun Kim
  • Byung Ju Lee

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)147-152
Journal / PublicationBiochemical and Biophysical Research Communications
Volume496
Issue number1
Online published4 Jan 2018
Publication statusPublished - 29 Jan 2018

Abstract

Here, we report thyroid transcription factor 1 (TTF-1) as an important transcription factor for the expression of heme oxygenase-1 (HO-1). HO-1 is a well-known cytoprotective enzyme against inflammation. We observed that HO-1 co-expressed with TTF-1 in mouse hypothalamic cells. Results from luciferase and chromatin immunoprecipitation assays revealed that TTF-1 directly activated HO-1 transcription by binding to binding domains in the 5'-flanking region of the HO-1 gene. A proinflammatory cytokine, tumor necrosis factor-alpha (TNF-α), induced nuclear translocation of TTF-1 and increased binding affinity of TTF-1 to its binding sites on the HO-1 gene. HO-1 mRNA increased with TTF-1 overexpression but decreased with RNA interference of TTF-1 expression in rat astroglial C6 cells. Together with results showing involvement of TTF-1 in the TNF-α-induced increase in interleukin 1 beta and monocyte chemotactic protein 1 production, this study suggests that TTF-1 plays an important role in the mouse hypothalamus TNF-α-induced inflammatory response for regulating HO-1 gene expression.

Research Area(s)

  • Astroglial activation, Heme oxygenase-1, Hypothalamic inflammation, Thyroid transcription factor-1

Citation Format(s)

Role of thyroid transcription factor-1 in transcriptional regulation of heme oxygenase-1. / Jeong, Bora; Kim, Han Rae; Choi, Nan Seong; Park, Byong Seo; Eom, Hyejin; Park, Jeong Woo; Kim, Jae Geun; Lee, Byung Ju.

In: Biochemical and Biophysical Research Communications, Vol. 496, No. 1, 29.01.2018, p. 147-152.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review