Role of NO and EDHF-mediated endothelial function in the porcine pulmonary circulation: Comparison between pulmonary artery and vein

Rong-Zhen Zhang; Qin Yang; Anthony P.C. Yim; Yu Huang; Guo-Wei He

doi:10.1016/j.vph.2005.11.005

Role of NO and EDHF-mediated endothelial function in the porcine pulmonary circulation: Comparison between pulmonary artery and vein

Rong-Zhen Zhang, Qin Yang, Anthony P.C. Yim, Yu Huang, Guo-Wei He

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

20 Citations (Scopus)

Abstract

Objective: To compare electrophysiological measurement of nitric oxide (NO) release and endothelium-derived hyperpolarizing factor (EDHF)-mediated endothelial function in porcine pulmonary arteries and veins. Methods: Isolated pulmonary interlobular arteries (PA) and veins (PV) were obtained from a local slaughterhouse. By using a NO-specific electrode and a conventional intracellular microelectrode, the amount of NO released from endothelial cells and hyperpolarization of smooth muscle cells were investigated. The bradykinin (BK)-induced relaxation in the precontraction by U₄₆₆₁₉ was examined in the absence or presence of N^G-nitro-l-arginine (l-NNA), indomethacin (INDO) plus oxyhemoglobin (HbO). Results: The basal release of NO was 7.0 ± 1.2 nmol/L in PA (n = 8) and 5.5 ± 1.6 nmol/L in PV (n = 8, p < 0.01). BK-induced release of NO was 160.4 ± 10.3 nmol/L in PA (n = 8) and 103.0 ± 14.7 nmol/L in PV (n = 8, p < 0.001) with longer releasing duration in PA than in PV (14.3 ± 1.3 vs. 12.1 ± 0.8 min, p < 0.01). BK evoked an endothelium-dependent hyperpolarization and relaxation that were reduced by l-NNA, INDO, and HbO (hyperpolarization: 12.8 ± 1.3 vs. 8.0 ± 1.4 mV in PA, n = 6, p < 0.001 and 8.3 ± 1.4 vs. 3.0 ± 0.8 mV in PV, n = 6, p < 0.001; relaxation: 92.8 ± 3.1% vs. 19.6 ± 11.1% in PA n = 8, p < 0.001 and 70.3 ± 7.9% vs. 6.0 ± 6.8% in PV, n = 8, p < 0.001). Both hyperpolarization (8.0 ± 1.4 vs. 3.0 ± 0.8 mV, p < 0.001) and relaxation (19.6 ± 11.1% vs. 6.0 ± 6.8%, p < 0.01) were greater in PA than in PV. Conclusions: Both NO and EDHF play an important role in regulation of porcine pulmonary arterial and venous tones. The more significant role of NO and EDHF is revealed in pulmonary arteries than in veins.

Original language	English
Pages (from-to)	183-191
Journal	Vascular Pharmacology
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/j.vph.2005.11.005
Publication status	Published - Mar 2006
Externally published	Yes

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Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Endothelial factors
Endothelial function
Membrane potential
Nitric oxide
Pulmonary circulation

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@article{e1d838efc3074840b8ef5fc6203a1719,

title = "Role of NO and EDHF-mediated endothelial function in the porcine pulmonary circulation: Comparison between pulmonary artery and vein",

abstract = "Objective: To compare electrophysiological measurement of nitric oxide (NO) release and endothelium-derived hyperpolarizing factor (EDHF)-mediated endothelial function in porcine pulmonary arteries and veins. Methods: Isolated pulmonary interlobular arteries (PA) and veins (PV) were obtained from a local slaughterhouse. By using a NO-specific electrode and a conventional intracellular microelectrode, the amount of NO released from endothelial cells and hyperpolarization of smooth muscle cells were investigated. The bradykinin (BK)-induced relaxation in the precontraction by U46619 was examined in the absence or presence of NG-nitro-l-arginine (l-NNA), indomethacin (INDO) plus oxyhemoglobin (HbO). Results: The basal release of NO was 7.0 ± 1.2 nmol/L in PA (n = 8) and 5.5 ± 1.6 nmol/L in PV (n = 8, p < 0.01). BK-induced release of NO was 160.4 ± 10.3 nmol/L in PA (n = 8) and 103.0 ± 14.7 nmol/L in PV (n = 8, p < 0.001) with longer releasing duration in PA than in PV (14.3 ± 1.3 vs. 12.1 ± 0.8 min, p < 0.01). BK evoked an endothelium-dependent hyperpolarization and relaxation that were reduced by l-NNA, INDO, and HbO (hyperpolarization: 12.8 ± 1.3 vs. 8.0 ± 1.4 mV in PA, n = 6, p < 0.001 and 8.3 ± 1.4 vs. 3.0 ± 0.8 mV in PV, n = 6, p < 0.001; relaxation: 92.8 ± 3.1% vs. 19.6 ± 11.1% in PA n = 8, p < 0.001 and 70.3 ± 7.9% vs. 6.0 ± 6.8% in PV, n = 8, p < 0.001). Both hyperpolarization (8.0 ± 1.4 vs. 3.0 ± 0.8 mV, p < 0.001) and relaxation (19.6 ± 11.1% vs. 6.0 ± 6.8%, p < 0.01) were greater in PA than in PV. Conclusions: Both NO and EDHF play an important role in regulation of porcine pulmonary arterial and venous tones. The more significant role of NO and EDHF is revealed in pulmonary arteries than in veins.",

keywords = "Endothelial factors, Endothelial function, Membrane potential, Nitric oxide, Pulmonary circulation",

author = "Rong-Zhen Zhang and Qin Yang and Yim, {Anthony P.C.} and Yu Huang and Guo-Wei He",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

year = "2006",

month = mar,

doi = "10.1016/j.vph.2005.11.005",

language = "English",

volume = "44",

pages = "183--191",

journal = "Vascular Pharmacology",

issn = "1537-1891",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Role of NO and EDHF-mediated endothelial function in the porcine pulmonary circulation

T2 - Comparison between pulmonary artery and vein

AU - Zhang, Rong-Zhen

AU - Yang, Qin

AU - Yim, Anthony P.C.

AU - Huang, Yu

AU - He, Guo-Wei

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2006/3

Y1 - 2006/3

N2 - Objective: To compare electrophysiological measurement of nitric oxide (NO) release and endothelium-derived hyperpolarizing factor (EDHF)-mediated endothelial function in porcine pulmonary arteries and veins. Methods: Isolated pulmonary interlobular arteries (PA) and veins (PV) were obtained from a local slaughterhouse. By using a NO-specific electrode and a conventional intracellular microelectrode, the amount of NO released from endothelial cells and hyperpolarization of smooth muscle cells were investigated. The bradykinin (BK)-induced relaxation in the precontraction by U46619 was examined in the absence or presence of NG-nitro-l-arginine (l-NNA), indomethacin (INDO) plus oxyhemoglobin (HbO). Results: The basal release of NO was 7.0 ± 1.2 nmol/L in PA (n = 8) and 5.5 ± 1.6 nmol/L in PV (n = 8, p < 0.01). BK-induced release of NO was 160.4 ± 10.3 nmol/L in PA (n = 8) and 103.0 ± 14.7 nmol/L in PV (n = 8, p < 0.001) with longer releasing duration in PA than in PV (14.3 ± 1.3 vs. 12.1 ± 0.8 min, p < 0.01). BK evoked an endothelium-dependent hyperpolarization and relaxation that were reduced by l-NNA, INDO, and HbO (hyperpolarization: 12.8 ± 1.3 vs. 8.0 ± 1.4 mV in PA, n = 6, p < 0.001 and 8.3 ± 1.4 vs. 3.0 ± 0.8 mV in PV, n = 6, p < 0.001; relaxation: 92.8 ± 3.1% vs. 19.6 ± 11.1% in PA n = 8, p < 0.001 and 70.3 ± 7.9% vs. 6.0 ± 6.8% in PV, n = 8, p < 0.001). Both hyperpolarization (8.0 ± 1.4 vs. 3.0 ± 0.8 mV, p < 0.001) and relaxation (19.6 ± 11.1% vs. 6.0 ± 6.8%, p < 0.01) were greater in PA than in PV. Conclusions: Both NO and EDHF play an important role in regulation of porcine pulmonary arterial and venous tones. The more significant role of NO and EDHF is revealed in pulmonary arteries than in veins.

AB - Objective: To compare electrophysiological measurement of nitric oxide (NO) release and endothelium-derived hyperpolarizing factor (EDHF)-mediated endothelial function in porcine pulmonary arteries and veins. Methods: Isolated pulmonary interlobular arteries (PA) and veins (PV) were obtained from a local slaughterhouse. By using a NO-specific electrode and a conventional intracellular microelectrode, the amount of NO released from endothelial cells and hyperpolarization of smooth muscle cells were investigated. The bradykinin (BK)-induced relaxation in the precontraction by U46619 was examined in the absence or presence of NG-nitro-l-arginine (l-NNA), indomethacin (INDO) plus oxyhemoglobin (HbO). Results: The basal release of NO was 7.0 ± 1.2 nmol/L in PA (n = 8) and 5.5 ± 1.6 nmol/L in PV (n = 8, p < 0.01). BK-induced release of NO was 160.4 ± 10.3 nmol/L in PA (n = 8) and 103.0 ± 14.7 nmol/L in PV (n = 8, p < 0.001) with longer releasing duration in PA than in PV (14.3 ± 1.3 vs. 12.1 ± 0.8 min, p < 0.01). BK evoked an endothelium-dependent hyperpolarization and relaxation that were reduced by l-NNA, INDO, and HbO (hyperpolarization: 12.8 ± 1.3 vs. 8.0 ± 1.4 mV in PA, n = 6, p < 0.001 and 8.3 ± 1.4 vs. 3.0 ± 0.8 mV in PV, n = 6, p < 0.001; relaxation: 92.8 ± 3.1% vs. 19.6 ± 11.1% in PA n = 8, p < 0.001 and 70.3 ± 7.9% vs. 6.0 ± 6.8% in PV, n = 8, p < 0.001). Both hyperpolarization (8.0 ± 1.4 vs. 3.0 ± 0.8 mV, p < 0.001) and relaxation (19.6 ± 11.1% vs. 6.0 ± 6.8%, p < 0.01) were greater in PA than in PV. Conclusions: Both NO and EDHF play an important role in regulation of porcine pulmonary arterial and venous tones. The more significant role of NO and EDHF is revealed in pulmonary arteries than in veins.

KW - Endothelial factors

KW - Endothelial function

KW - Membrane potential

KW - Nitric oxide

KW - Pulmonary circulation

UR - http://www.scopus.com/inward/record.url?scp=31844440722&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-31844440722&origin=recordpage

U2 - 10.1016/j.vph.2005.11.005

DO - 10.1016/j.vph.2005.11.005

M3 - RGC 21 - Publication in refereed journal

C2 - 16448859

SN - 1537-1891

VL - 44

SP - 183

EP - 191

JO - Vascular Pharmacology

JF - Vascular Pharmacology

IS - 3

ER -

Role of NO and EDHF-mediated endothelial function in the porcine pulmonary circulation: Comparison between pulmonary artery and vein

Abstract

Bibliographical note

Research Keywords

UN SDGs

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