Robust Nitritation Sustained by Acid-Tolerant Ammonia-Oxidizing Bacteria

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Zhiyao Wang
  • Min Zheng
  • Jia Meng
  • Zhetai Hu
  • Gaofeng Ni
  • Angelica Guerrero Calderon
  • Huijuan Li
  • Haydee De Clippeleir
  • Ahmed Al-Omari
  • Shihu Hu

Detail(s)

Original languageEnglish
Pages (from-to)2048-2056
Journal / PublicationEnvironmental Science and Technology
Volume55
Issue number3
Online published14 Jan 2021
Publication statusPublished - 2 Feb 2021
Externally publishedYes

Abstract

Oxidation of ammonium to nitrite rather than nitrate, i.e., nitritation, is critical for autotrophic nitrogen removal. This study demonstrates a robust nitritation process in treating low-strength wastewater, obtained from a mixture of real mainstream sewage with sidestream anaerobic digestion liquor. This is achieved through cultivating acid-tolerant ammonia-oxidizing bacteria (AOB) in a laboratory nitrifying bioreactor at pH 4.5-5.0. It was shown that nitrite accumulation with a high NO2-/(NO2- + NO3-) ratio of 95 ± 5% was stably maintained for more than 300 days, and the obtained volumetric NH4+ removal rate (i.e., 188 ± 14 mg N L-1 d-1) was practically useful. 16S rRNA gene sequencing analyses indicated the dominance of new AOB, "Candidatus Nitrosoglobus,"in the nitrifying guild (i.e., 1.90 ± 0.08% in the total community), with the disappearance of typical activated sludge nitrifying microorganisms, including Nitrosomonas, Nitrospira, and Nitrobacter. This is the first identification of Ca. Nitrosoglobus as key ammonia oxidizers in a wastewater treatment system. It was found that Ca. Nitrosoglobus can tolerate low pH (<5.0), and free nitrous acid (FNA) at levels that inhibit AOB and nitrite-oxidizing bacteria (NOB) commonly found in wastewater treatment processes. The in situ inhibition of NOB leads to accumulation of nitrite (NO2-), which along with protons (H+) also produced in ammonium oxidation generates and sustains FNA at 3.0 ± 1.4 mg HNO2-N L-1. As such, robust PN was achieved under acidic conditions, with a complete absence of NOB. Compared to previous nitritation systems, this acidic nitritation process is featured by a higher nitric oxide (NO) but a lower nitrous oxide (N2O) emission level, with the emission factors estimated at 1.57 ± 0.08 and 0.57 ± 0.03%, respectively, of influent ammonium nitrogen load. ©  2021 American Chemical Society.

Citation Format(s)

Robust Nitritation Sustained by Acid-Tolerant Ammonia-Oxidizing Bacteria. / Wang, Zhiyao; Zheng, Min; Meng, Jia et al.
In: Environmental Science and Technology, Vol. 55, No. 3, 02.02.2021, p. 2048-2056.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review