RNA G-quadruplex structures exist and function in vivo in plants
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Article number | 226 |
Journal / Publication | Genome Biology |
Volume | 21 |
Online published | 1 Sept 2020 |
Publication status | Published - 2020 |
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DOI | DOI |
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Attachment(s) | Documents
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85090180550&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(63a64a40-bcd0-402d-8e10-69ac4dc0d90a).html |
Abstract
Background: Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct evidence for RNA G-quadruplex formation in living eukaryotic cells. Therefore, it is unclear whether any purported functions are associated with the specific sequence content or the formation of an RNA G-quadruplex structure. Results: Using rG4-seq, we profile the landscape of those guanine-rich regions with the in vitro folding potential in the Arabidopsis transcriptome. We find a global enrichment of RNA G-quadruplexes with two G-quartets whereby the folding potential is strongly influenced by RNA secondary structures. Using in vitro and in vivo RNA chemical structure profiling, we determine that hundreds of RNA G-quadruplex structures are strongly folded in both Arabidopsis and rice, providing direct evidence of RNA G-quadruplex formation in living eukaryotic cells. Subsequent genetic and biochemical analyses show that RNA G-quadruplex folding is able to regulate translation and modulate plant growth. Conclusions: Our study reveals the existence of RNA G-quadruplex in vivo and indicates that RNA G-quadruplex structures act as important regulators of plant development and growth.
Research Area(s)
- Gene regulation, Plant development, rG4-seq, RNA G-quadruplex structure, Selective 2′-hydroxyl acylation with lithium ion-based primer extension (SHALiPE)
Citation Format(s)
RNA G-quadruplex structures exist and function in vivo in plants. / Yang, Xiaofei; Cheema, Jitender; Zhang, Yueying et al.
In: Genome Biology, Vol. 21, 226, 2020.
In: Genome Biology, Vol. 21, 226, 2020.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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