Risk factors for Neospora caninum, bovine viral diarrhoea virus, and Leptospira interrogans serovar hardjo infection in smallholder cattle and buffalo in Lao PDR

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

View graph of relations

Author(s)

  • Luisa Olmo
  • Sonevilay Nampanya
  • Syseng Khounsy
  • Lloyd C. Wahl
  • Bethanie A. Clark
  • Peter C. Thomson
  • Peter A. Windsor
  • Russell D. Bush

Detail(s)

Original languageEnglish
Article numbere0220335
Journal / PublicationPLoS ONE
Volume14
Issue number8
Online published8 Aug 2019
Publication statusPublished - 2019

Link(s)

Abstract

Smallholder large ruminant production in Lao People’s Democratic Republic (Laos) is characterised by low reproductive efficiency. To determine if common abortifacient bovid infectious diseases are involved, a serological investigation was conducted. Sera was collected from stored and fresh cattle (n = 390) and buffalo (n = 130) samples from 2016–18 from, and then examined for associations in a retrospective risk factor study of 71 herds. The sera were assayed for antibodies to Neospora caninum, bovine viral diarrhoea virus (BVDV), Leptospira interrogans serovar Hardjo and Brucella abortus using commercially available enzyme-linked immunosorbent assay kits. These pathogens were detected in buffalo samples at 78.5% (95% CI 71.4–85.6), 0%, 2.3% (95% CI 0–4.9) and 0%, respectively, and in cattle at 4.4% (95% CI 2.4–6.4), 7.7% (95% CI 3.1–12.3), 12.8% (95% CI 9.5–16.1) and 0.26% (95% CI 0–0.8), respectively. Exposure of buffalo to Ncaninum was positively associated with buffalo age, with a predicted seropositivity at birth of 52.8%, increasing to 97.2% by 12 years of age (p = 0.037). Exposure of cattle to Linterrogans serovar Hardjo was more prevalent in females compared to males, was associated with higher titres of BVDV, and was more prevalent in the wet season compared to the dry season. Exposure of cattle to BVDV was more prevalent in males compared to females, the wet and dry seasons were comparable, and was associated with rising antibody titres against Ncaninum and Linterrogans serovar Hardjo. The risk factor survey identified that the probability of herds being Ncaninum positive increased with farmer age, if farmers believed there were rodents on farm, and if farmers weren’t aware that canids or rodents could contaminate bovid feed on their farm. The probability of a herd being positive to Linterrogans serovar Hardjo increased on farms where multiple cows shared the same bull, where farmers had lower husbandry knowledge, and on farms that used water troughs. The probability of a herd being BVDV seropositive increased with increasing herd size and increasing titres to Ncaninum. The benchmarking of bovid exposure to emerging abortifacient pathogens and identification of their risk factors potentially informs disease prevention strategies, supporting efforts to establish a biosecure beef supply for enhanced smallholder livestock productivity, public health and food security in Laos and surrounding countries.

Research Area(s)

Citation Format(s)

Risk factors for Neospora caninum, bovine viral diarrhoea virus, and Leptospira interrogans serovar hardjo infection in smallholder cattle and buffalo in Lao PDR. / Olmo, Luisa; Reichel, Michael P.; Nampanya, Sonevilay; Khounsy, Syseng; Wahl, Lloyd C.; Clark, Bethanie A.; Thomson, Peter C.; Windsor, Peter A.; Bush, Russell D.

In: PLoS ONE, Vol. 14, No. 8, e0220335, 2019.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

Download Statistics

No data available