RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome

Chun Kit Kwok; Giovanni Marsico; Aleksandr B Sahakyan; Vicki S Chambers; Shankar Balasubramanian

doi:10.1038/nmeth.3965

Author(s)

Chun Kit Kwok
Giovanni Marsico
Aleksandr B Sahakyan
Vicki S Chambers
Shankar Balasubramanian

Detail(s)

Original language	English
Pages (from-to)	841-844
Journal / Publication	Nature Methods
Volume	13
Issue number	10
Publication status	Published - 1 Oct 2016
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1038/nmeth.3965 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84984611742&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(23543c9e-98ea-4b13-92a2-38bb1bc1f24b).html

Abstract

We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.

Citation Format(s)

[ RIS ] [ BibTeX ]

RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. / Kwok, Chun Kit; Marsico, Giovanni; Sahakyan, Aleksandr B et al.

In: Nature Methods, Vol. 13, No. 10, 01.10.2016, p. 841-844.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

Kwok, CK, Marsico, G, Sahakyan, AB, Chambers, VS & Balasubramanian, S 2016, 'RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome', Nature Methods, vol. 13, no. 10, pp. 841-844. https://doi.org/10.1038/nmeth.3965

Kwok, C. K., Marsico, G., Sahakyan, A. B., Chambers, V. S., & Balasubramanian, S. (2016). RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. Nature Methods, 13(10), 841-844. https://doi.org/10.1038/nmeth.3965

Kwok CK, Marsico G, Sahakyan AB, Chambers VS, Balasubramanian S. RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. Nature Methods. 2016 Oct 1;13(10):841-844. doi: 10.1038/nmeth.3965

Kwok, Chun Kit ; Marsico, Giovanni ; Sahakyan, Aleksandr B et al. / RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. In: Nature Methods. 2016 ; Vol. 13, No. 10. pp. 841-844.