RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chun Kit Kwok
  • Giovanni Marsico
  • Aleksandr B Sahakyan
  • Vicki S Chambers
  • Shankar Balasubramanian

Detail(s)

Original languageEnglish
Pages (from-to)841-844
Journal / PublicationNature Methods
Volume13
Issue number10
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Abstract

We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.

Citation Format(s)

RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome. / Kwok, Chun Kit; Marsico, Giovanni; Sahakyan, Aleksandr B; Chambers, Vicki S; Balasubramanian, Shankar.

In: Nature Methods, Vol. 13, No. 10, 01.10.2016, p. 841-844.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review