TY - JOUR
T1 - RG4-seq reveals widespread formation of G-quadruplex structures in the human transcriptome
AU - Kwok, Chun Kit
AU - Marsico, Giovanni
AU - Sahakyan, Aleksandr B
AU - Chambers, Vicki S
AU - Balasubramanian, Shankar
PY - 2016/10/1
Y1 - 2016/10/1
N2 - We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.
AB - We introduce RNA G-quadruplex sequencing (rG4-seq), a transcriptome-wide RNA G-quadruplex (rG4) profiling method that couples rG4-mediated reverse transcriptase stalling with next-generation sequencing. Using rG4-seq on polyadenylated-enriched HeLa RNA, we generated a global in vitro map of thousands of canonical and noncanonical rG4 structures. We characterize rG4 formation relative to cytosine content and alternative RNA structure stability, uncover rG4-dependent differences in RNA folding and show evolutionarily conserved enrichment in transcripts mediating RNA processing and stability.
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U2 - 10.1038/nmeth.3965
DO - 10.1038/nmeth.3965
M3 - RGC 21 - Publication in refereed journal
SN - 1548-7091
VL - 13
SP - 841
EP - 844
JO - Nature Methods
JF - Nature Methods
IS - 10
ER -