Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Kyu Tae Lee
  • Seongjin Hong
  • Jung Suk Lee
  • Kyu Hyuck Chung
  • Klara Hilscherová
  • And 2 others
  • John P. Giesy
  • Jong Seong Khim

Detail(s)

Original languageEnglish
Pages (from-to)8590-8599
Journal / PublicationEnvironmental Science and Pollution Research
Volume20
Issue number12
Publication statusPublished - 2013

Abstract

While the World Health Organization 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) equivalency factors are useful estimates of relative potencies of mixtures when conducting risk assessments, they are not useful when comparing the results of bioassays such as the H4IIE-luc to concentrations of TCDD equivalents calculated from instrumental analyses. Since there are thousands of dioxin-like compounds (DLCs), one use of screening assays is to determine if all of the aryl hydrocarbon receptor (AhR) active DLCs in a mixture have been accounted for in instrumental analyses. For this purpose, bioassay-specific relative potency (ReP) values are needed. RePs of 21 polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and dioxin-like polychlorinated biphenyls that exhibit effects mediated through the AhR were determined by use of the H4IIE-luc assay. Different values of RePs are derived, depending on the statistical, curve-fitting methods used to derive them from the dose-response relationships. Here, we discuss the various methods for deriving RePs from in vitro data and their assumptions and effects on values of RePs. Full dose-response curves of 2,3,7,8-TCDD and other representative DLCs were used to estimate effective concentrations at multiple points (e.g., EC20-50-80), which were then used to estimate ReP of each DLC to 2,3,7,8-TCDD. © 2013 Springer-Verlag Berlin Heidelberg.

Research Area(s)

  • AhR activity, Dioxin-like compounds, In vitro bioassay, Relative potency, Toxic equivalency factor

Citation Format(s)

Revised relative potency values for PCDDs, PCDFs, and non-ortho-substituted PCBs for the optimized H4IIE-luc in vitro bioassay. / Lee, Kyu Tae; Hong, Seongjin; Lee, Jung Suk; Chung, Kyu Hyuck; Hilscherová, Klara; Giesy, John P.; Khim, Jong Seong.

In: Environmental Science and Pollution Research, Vol. 20, No. 12, 2013, p. 8590-8599.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review