Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

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Author(s)

  • Junjie Yao
  • Andrii A. Kaberniuk
  • Lei Li
  • Daria M. Shcherbakova
  • Ruiying Zhang
  • Guo Li
  • Vladislav V. Verkhusha
  • Lihong V. Wanga

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume9708
ISBN (Print)9781628419429
Publication statusPublished - 2016
Externally publishedYes

Publication series

Name
Volume9708
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2016
PlaceUnited States
CitySan Francisco
Period14 - 17 February 2016

Abstract

Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ∼2 μM, or as few as ∼20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ∼100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ∼140 nm and axial resolution of ∼400 nm, which are respectively ∼2-fold and ∼75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.

Research Area(s)

  • Bacterial phytochrome, Deep tissue imaging, Genetic imaging, Near-infrared protein, Photoacoustic microscopy, Photoacoustic tomography, Reversibly switchable protein, Super-resolution imaging

Citation Format(s)

Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. / Yao, Junjie; Kaberniuk, Andrii A.; Li, Lei et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 9708 SPIE, 2016. 97082U.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review