Reversibly Photoswitching Upconversion Nanoparticles for Super-Sensitive Photoacoustic Molecular Imaging
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
Original language | English |
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Article number | e202116802 |
Journal / Publication | Angewandte Chemie (International Edition) |
Volume | 61 |
Issue number | 19 |
Online published | 9 Feb 2022 |
Publication status | Published - 2 May 2022 |
Link(s)
Abstract
Photoacoustic (PA) imaging uses light excitation to generate the acoustic signal for detection and improves tissue penetration depth and spatial resolution in the clinically relevant depth of living subjects. However, strong background signals from blood and pigments have significantly compromised the sensitivity of PA imaging with exogenous contrast agents. Here we report a nanoparticle-based probe design that uses light to reversibly modulate the PA emission to enable photoacoustic photoswitching imaging (PAPSI) in living mice. Such a nanoprobe is built with upconverting nanocrystals and photoswitchable small molecules and can be switched on by NIR light through upconversion to UV energy. Reversibly photoswitching of the nanoprobe reliably removed strong tissue background, increased the contrast-to-noise ratio, and thus improved imaging sensitivity. We have shown that PAPSI can image 0.05 nM of the nanoprobe in hemoglobin solutions and 104 labeled cancer cells after implantation in living mice using a commercial PA imager.
Research Area(s)
- Imaging Agents, Near-infrared, Photoacoustic Imaging, Reversible Photoswitching, Upconversion Nanoparticles, SEMICONDUCTING POLYMER NANOPARTICLES, TOMOGRAPHY, MICROSCOPY, RECEPTOR, AGENT
Citation Format(s)
Reversibly Photoswitching Upconversion Nanoparticles for Super-Sensitive Photoacoustic Molecular Imaging. / Liu, Cheng; Zheng, Xianchuang; Dai, Tingting et al.
In: Angewandte Chemie (International Edition), Vol. 61, No. 19, e202116802, 02.05.2022.
In: Angewandte Chemie (International Edition), Vol. 61, No. 19, e202116802, 02.05.2022.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review