Response of the reactor performance and microbial community to a shift of ISDD process from micro-aerobic to anoxic condition

Micro-aerobic condition has proven to be effective in enhancing sulfide oxidation to elemental sulfur (S⁰) during integrated simultaneous desulfurization and denitrification process (ISDD). In this study we investigated and compared the performance and microbial community of ISDD process operating under initially anoxic, then micro-aerobic and finally switch back to anoxic condition. For all the three tested scenarios, comparable bioreactor performance in terms of sulfate (95.0 ± 4.4%, 90.6 ± 3.8%, 89.8 ± 3.5%) and nitrate (∼100%) removal was achieved. However, a shift of ISDD bioreactor from micro-aerobic to anoxic environment clearly increased the S⁰ production (30.6%), relative to that at initial anoxic condition (14.2%). Further anoxic bioreactor operation with different influent nitrate concentrations also obtained satisfactory performance particularly in terms of S⁰ production. Microbial community analysis results showed that functional microorganisms selectively enriched at micro-aerobic condition, particularly sulfide-oxidizing bacteria (SOB), could also function well and enhance S⁰ production when bioreactor switching from micro-aerobic to anoxic environment. We proposed that micro-aerobic strategy could function as a bio-selector and provide a new idea in functional microorganisms selectively enrichment for wastewater treatment.