Response of 5 hpf zebrafish embryos to low-dose microbeam protons

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Viann Wing Yan Choi
  • Candy Yuen Ping NG
  • Alisa KOBAYASHI
  • Teruaki KONISHI
  • Masakazu OIKAWA

Detail(s)

Original languageEnglish
Pages (from-to)i113
Journal / PublicationJournal of Radiation Research
Volume55
Issue numbersuppl_1
Online published1 Mar 2014
Publication statusPublished - Mar 2014

Link(s)

Abstract

The microbeam irradiation system (single-particle irradiation system to cell, acronym as SPICE) at the National Institute of Radiological Sciences (NIRS), Japan, was employed to irradiate dechorionated embryos of the zebrafish, Danio rerio, at 5 h post-fertilization (hpf) by protons each having an energy of ∼3.4 MeV. Either 1 or 10 positions on the cells were irradiated with different number of protons. The levels of apoptosis in zebrafish embryos at 25 hpf were quantified through terminal dUTP transferase-mediated nick end-labeling assay. Triphasic dose–esponses were obtained (Fig. 1), including (i) a subhormetic zone with an increase in apoptotic signals for a small number of irradiated protons per position, (ii) a hormetic zone with a reduction in the apoptotic signals below the spontaneous level for a larger number of irradiated protons per position and (iii) a toxic zone with an increase in apoptotic signals again if the number of irradiated protons per position was further increased.

Research Area(s)

Citation Format(s)

Response of 5 hpf zebrafish embryos to low-dose microbeam protons. / Choi, Viann Wing Yan; NG, Candy Yuen Ping; KOBAYASHI, Alisa; KONISHI, Teruaki; OIKAWA, Masakazu; CHENG, Shuk Han; YU, Kwan Ngok.

In: Journal of Radiation Research, Vol. 55, No. suppl_1, 03.2014, p. i113.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review