Research progress and prospects of experimental murine behavior evaluation methods

Experimental murine behaviors, including communication, emotional expression, social interaction, learning, and reproduction, play a critical role in various research fields such as genetics, neuroscience, and non-neuroscience disciplines. The growing demand for murine behavior research has driven the development of diverse evaluation methods. Current evaluation techniques include assessments based on ordinary or high-speed cameras, active depth sensors, inertial sensors, optical pressure plates, electromyography, and two-photon fluorescence microscopy. Each method has its unique advantages and limitations depending on the experimental context. Therefore, selecting an appropriate evaluation method tailored to specific experimental requirements is crucial for obtaining accurate and meaningful results. This review summarizes the advancements in experimental murine behavior evaluation methods over the past decade, providing a comprehensive overview of their applications and contributions to behavioral research. © 2025 The Authors.