Requirement of IP3 receptor 3 (IP3R3) in nitric oxide induced cardiomyocyte differentiation of mouse embryonic stem cells
Related Research Unit(s)
|Journal / Publication||Experimental Cell Research|
|Online published||24 Jun 2016|
|Publication status||Published - 1 Aug 2016|
|Link to Scopus||https://www.scopus.com/record/display.uri?eid=2-s2.0-84979703421&origin=recordpage|
Nitric oxide (NO) markedly induces cardiomyocyte (CM) differentiation of embryonic stem (ES) cells. Here we examined the role of the Ca2+ signaling in the NO-induced CM differentiation of mouse ES cells. We found that NO induced intracellular Ca2+ increases in ES cells in a dose-dependent manner, and application of IP3 pathway antagonists not only significantly inhibited this induced Ca2+ increase but also abolished NO-induced CM differentiation of ES cells. Subsequently, all 3 types of inositol 1, 4, 5-trisphosphate (IP3) receptors (IP3Rs) in mouse ES cells were individually or triply knocked down. Interestingly, only knockdown of type 3 IP3R (IP3R3) or triple-knockdown of three types of IP3Rs significantly inhibited the NO-induced Ca2+ increases. Consistently, IP3R3 knockdown blocked the NO-induced CM differentiation of ES cells. CMs derived from IP3R3 knockdown ES cells also showed both structural and functional defects. In summary, our results indicate that the IP3R3-Ca2+ pathway is required for NO-induced CM differentiation of ES cells.
- Ca2+, Cardiomyocyte, ES cells, IP3 receptor 3, Nitric oxide
Experimental Cell Research, Vol. 346, No. 1, 01.08.2016, p. 9-16.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal
Wei, W, Huang, W & Yue, J 2016, 'Requirement of IP3 receptor 3 (IP3R3) in nitric oxide induced cardiomyocyte differentiation of mouse embryonic stem cells', Experimental Cell Research, vol. 346, no. 1, pp. 9-16. https://doi.org/10.1016/j.yexcr.2016.06.016