Repeated capillary electrophoresis separations conducted on a commercial DNA chip

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Detail(s)

Original languageEnglish
Pages (from-to)864-868
Journal / PublicationAnalytical Methods
Volume4
Issue number3
Publication statusPublished - Mar 2012
Externally publishedYes

Abstract

Multi-sample microchip capillary electrophoresis separations of a DNA sample (i.e. pUC18 DNA Hae III restriction digest) have been conducted on a commercial bioanalyzer. According to the vendor's specifications, a new chip must be used and the gel-dye mix must be used within 2 weeks for the best possible results. However, such a requirement of new chips and fresh reagents is quite costly, especially during preliminary research work, or instructional experiments in teaching labs. Therefore, we have developed a method to clean the microchip, so that the same DNA chip can be inspected and re-used. Our experimental data show that the CE separations conducted on a new chip and on a cleaned chip are comparable without loss in sizing accuracy and reproducibility. The gel-dye mix can also be used many times, longer than 2 weeks as specified by the vendor until the gel-dye changes color. This method will provide cost-saving advantage to laboratories during training and preliminary research work. © 2012 The Royal Society of Chemistry.

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