Relevance of TRPA1 and TRPM8 channels as vascular sensors of cold in the cutaneous microvasculature

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • D. Thapa
  • L. Baldissera Jr
  • F. Argunhan
  • A. A. Aubdool
  • S. D. Brain

Detail(s)

Original languageEnglish
Pages (from-to)779-786
Number of pages8
Journal / PublicationPflugers Archiv European Journal of Physiology
Volume470
Issue number5
Online published21 Nov 2017
Publication statusPublished - May 2018
Externally publishedYes

Link(s)

Abstract

Cold exposure is directly related to skin conditions, such as frostbite. This is due to the cold exposure inducing a vasoconstriction to reduce cutaneous blood flow and protect against heat loss. However, a long-term constriction will cause ischaemia and potentially irreversible damage. We have developed techniques to elucidate the mechanisms of the vascular cold response. We focused on two ligand-gated transient receptor potential (TRP) channels, namely, the established "cold sensors" TRP ankyrin 1 (TRPA1) and TRP melastin (TRPM8). We used the anaesthetised mouse and measured cutaneous blood flow by laser speckle imaging. Two cold treatments were used. A generalised cold treatment was achieved through whole paw water immersion (10 °C for 5 min) and a localised cold treatment that will be potentially easier to translate to human studies was carried out on the mouse paw with a copper cold probe (0.85-cm diameter). The results show that TRPA1 and TRPM8 can each act as a vascular cold sensor to mediate the vasoconstrictor component of whole paw cooling as expected from our previous research. However, the local cooling-induced responses were only blocked when the TRPA1 and TRPM8 antagonists were given simultaneously. This suggests that this localised cold probe response requires both functional TRPA1 and TRPM8.

© The Author(s) 2017.

Research Area(s)

  • Animals, Cold Temperature, Male, Mice, Microvessels/metabolism, Skin/blood supply, TRPA1 Cation Channel/metabolism, TRPM Cation Channels/metabolism, Thermosensing, Vasoconstriction

Citation Format(s)

Relevance of TRPA1 and TRPM8 channels as vascular sensors of cold in the cutaneous microvasculature. / Pan, Y.; Thapa, D.; Baldissera Jr, L. et al.
In: Pflugers Archiv European Journal of Physiology, Vol. 470, No. 5, 05.2018, p. 779-786.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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