Regulation of extracellular bioactive cations in bone tissue microenvironment induces favorable osteoimmune conditions to accelerate in situ bone regeneration

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Zhengjie Lin
  • Danni Shen
  • Weixiao Zhou
  • Yufeng Zheng
  • Tiantian Kong
  • Xuanyong Liu
  • Shuilin Wu
  • Ying Zhao
  • Jun Wu
  • Kenneth M.C. Cheung
  • Kelvin W.K. Yeung

Detail(s)

Original languageEnglish
Pages (from-to)2315-2330
Journal / PublicationBioactive Materials
Volume6
Issue number8
Online published23 Jan 2021
Publication statusPublished - Aug 2021

Link(s)

Abstract

The design of orthopedic biomaterials has gradually shifted from “immune-friendly” to “immunomodulatory,” in which the biomaterials are able to modulate the inflammatory response via macrophage polarization in a local immune microenvironment that favors osteogenesis and implant-to-bone osseointegration. Despite the well-known effects of bioactive metallic ions on osteogenesis, how extracellular metallic ions manipulate immune cells in bone tissue microenvironments toward osteogenesis and subsequent bone formation has rarely been studied. Herein, we investigate the osteoimmunomodulatory effect of an extracellular bioactive cation (Mg2+) in the bone tissue microenvironment using custom-made poly lactic-co-glycolic acid (PLGA)/MgO-alendronate microspheres that endow controllable release of magnesium ions. The results suggest that the Mg2+-controlled tissue microenvironment can effectively induce macrophage polarization from the M0 to M2 phenotype via the enhancement of anti-inflammatory (IL-10) and pro-osteogenic (BMP-2 and TGF-β1) cytokines production. It also generates a favorable osteoimmune microenvironment that facilitates the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. The in vivo results further verify that a large amount of bony tissue, with comparable bone mineral density and mechanical properties, has been generated at an early post-surgical stage in rat intramedullary bone defect models. This study demonstrates that the concept of in situ immunomodulated osteogenesis can be realized in a controlled magnesium tissue microenvironment.

Research Area(s)

  • Bone regeneration, Macrophage polarization, Magnesium ions, Osteoimmune environment, Osteoimmunomodulatory property

Citation Format(s)

Regulation of extracellular bioactive cations in bone tissue microenvironment induces favorable osteoimmune conditions to accelerate in situ bone regeneration. / Lin, Zhengjie; Shen, Danni; Zhou, Weixiao; Zheng, Yufeng; Kong, Tiantian; Liu, Xuanyong; Wu, Shuilin; Chu, Paul K.; Zhao, Ying; Wu, Jun; Cheung, Kenneth M.C.; Yeung, Kelvin W.K.

In: Bioactive Materials, Vol. 6, No. 8, 08.2021, p. 2315-2330.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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