Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

16 Scopus Citations
View graph of relations

Author(s)

  • Suraia Nusrin
  • Steve K.H. Tong
  • G. Chaturvedi
  • Rudolf S.S. Wu
  • John P. Giesy

Detail(s)

Original languageEnglish
Pages (from-to)344-351
Journal / PublicationMarine Pollution Bulletin
Volume85
Issue number2
Online published24 Apr 2014
Publication statusPublished - 30 Aug 2014

Abstract

Although numerous studies have shown that hypoxia affects cortisol and aldosterone production in vivo, the underlying molecular mechanisms regulating the steroidogenic genes of these steroid hormones are still poorly known. MicroRNAs are post-transcriptional regulators that control diverse biological processes and this study describes the identification and validation of the hypoxia-inducible microRNA, miR-10b, as a negative regulator of the CYP11B1 and CYP11B2 steroidogenic genes in H295R human adrenocortical cells. Using the human TaqMan Low Density miRNA Arrays, we determined the miRNA expression patterns in H295R cells under normoxic and hypoxic conditions, and in cells overexpressing the human HIF-1α. Computer analysis using three in silico algorithms predicted that the hypoxia-inducible miR-10b molecule targets CYP11B1 and CYP11B2 mRNAs. Gene transfection studies of luciferase constructs containing the 3'-untranslated region of CYP11B1 or CYP11B2, combined with miRNA overexpression and knockdown experiments provide compelling evidence that CYP11B1 and CYP11B2 mRNAs are likely targets of miR-10b. © 2014 Elsevier Ltd.

Research Area(s)

  • Aldosterone, Cortisol, CYP11B1, CYP11B2, Hypoxia-inducible factor, MiR-10b

Citation Format(s)

Regulation of CYP11B1 and CYP11B2 steroidogenic genes by hypoxia-inducible miR-10b in H295R cells. / Nusrin, Suraia; Tong, Steve K.H.; Chaturvedi, G.; Wu, Rudolf S.S.; Giesy, John P.; Kong, Richard Y.C.

In: Marine Pollution Bulletin, Vol. 85, No. 2, 30.08.2014, p. 344-351.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review