Refinement of a colostrum-deprived pig model for infectious disease research

Tanja Opriessnig; Priscilla F. Gerber; Patrick G. Halbur

doi:10.1016/j.mex.2018.03.010

Refinement of a colostrum-deprived pig model for infectious disease research

Tanja Opriessnig^*
, Priscilla F. Gerber
, Patrick G. Halbur

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

7 Citations (Scopus)

16 Downloads (CityUHK Scholars)

Abstract

Well-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the easiness of getting pigs in a timely manner. However, freedom of the pig for the pathogen of interest is important at study start and serological assays to screen pigs for antibodies against newly identified pathogens or molecular assays detecting all possible circulating pathogen variants may not yet exist. Using colostrum-deprived (CD) pigs is a good alternative strategy to circumvent passively-acquired immunity against the pathogen of interest or exposure to pathogens shortly after birth. However, CD pigs are difficult to rear as they are highly susceptible to infections, and mortality rates in the first few days of life are often very high. Herein we report on refinement of a CD pig model with consistent survival rates of 90–100% of the piglets. • Step-by-step protocol to derive and rear CD piglets with higher expected survival rates.• Pig housing improvement minimizes the risk of disease transmission.• Infectious virus disease research pig model purpose.

Original language	English
Pages (from-to)	403-413
Journal	MethodsX
Volume	5
DOIs	https://doi.org/10.1016/j.mex.2018.03.010
Publication status	Published - 1 Jan 2018
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]

Funding

Funding was received by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant awarded to the Roslin Institute (BB/J004324/1; BBS/E/D/20241864).

Research Keywords

Artificially-reared
Colostrum-deprived pigs
Infectious pathogens
Naturally-farrowed
Pig model

Publisher's Copyright Statement

This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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title = "Refinement of a colostrum-deprived pig model for infectious disease research",

abstract = "Well-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the easiness of getting pigs in a timely manner. However, freedom of the pig for the pathogen of interest is important at study start and serological assays to screen pigs for antibodies against newly identified pathogens or molecular assays detecting all possible circulating pathogen variants may not yet exist. Using colostrum-deprived (CD) pigs is a good alternative strategy to circumvent passively-acquired immunity against the pathogen of interest or exposure to pathogens shortly after birth. However, CD pigs are difficult to rear as they are highly susceptible to infections, and mortality rates in the first few days of life are often very high. Herein we report on refinement of a CD pig model with consistent survival rates of 90–100\% of the piglets. • Step-by-step protocol to derive and rear CD piglets with higher expected survival rates.• Pig housing improvement minimizes the risk of disease transmission.• Infectious virus disease research pig model purpose.",

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AU - Halbur, Patrick G.

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N2 - Well-defined pig models are useful to study the pathogenicity of newly recognized pathogens or strains in pigs and serve as animal models for some human diseases. The conventional pig model, where research pigs are sourced from commercial high-health production systems, is commonly used due to the easiness of getting pigs in a timely manner. However, freedom of the pig for the pathogen of interest is important at study start and serological assays to screen pigs for antibodies against newly identified pathogens or molecular assays detecting all possible circulating pathogen variants may not yet exist. Using colostrum-deprived (CD) pigs is a good alternative strategy to circumvent passively-acquired immunity against the pathogen of interest or exposure to pathogens shortly after birth. However, CD pigs are difficult to rear as they are highly susceptible to infections, and mortality rates in the first few days of life are often very high. Herein we report on refinement of a CD pig model with consistent survival rates of 90–100% of the piglets. • Step-by-step protocol to derive and rear CD piglets with higher expected survival rates.• Pig housing improvement minimizes the risk of disease transmission.• Infectious virus disease research pig model purpose.

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JO - MethodsX

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Refinement of a colostrum-deprived pig model for infectious disease research

Abstract

Bibliographical note

Funding

Research Keywords

Publisher's Copyright Statement

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