Red Blood Cell-Derived Extracellular Vesicles Display Endogenous Antiviral Effects and Enhance the Efficacy of Antiviral Oligonucleotide Therapy
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Pages (from-to) | 21639-21661 |
Journal / Publication | ACS Nano |
Volume | 17 |
Issue number | 21 |
Online published | 18 Oct 2023 |
Publication status | Published - 14 Nov 2023 |
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DOI | DOI |
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Attachment(s) | Documents
Publisher's Copyright Statement
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85177167565&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(6bde69ef-1ff1-400d-830c-e42caeddeef1).html |
Abstract
The COVID-19 pandemic has resulted in a large number of fatalities and, at present, lacks a readily available curative treatment for patients. Here, we demonstrate that unmodified red blood cell-derived extracellular vesicles (RBCEVs) can inhibit SARS-CoV-2 infection in a phosphatidylserine (PS) dependent manner. Using T cell immunoglobulin mucin domain-1 (TIM-1) as an example, we demonstrate that PS receptors on cells can significantly increase the adsorption and infection of authentic and pseudotyped SARS-CoV-2 viruses. RBCEVs competitively inhibit this interaction and block TIM-1-mediated viral entry into cells. We further extend the therapeutic efficacy of this antiviral treatment by loading antisense oligonucleotides (ASOs) designed to target conserved regions of key SARS-CoV-2 genes into RBCEVs. We establish that ASO-loaded RBCEVs are efficiently taken up by cells in vitro and in vivo to suppress SARS-CoV-2 replication. Our findings indicate that this RBCEV-based SARS-CoV-2 therapeutic displays promise as a potential treatment capable of inhibiting SARS-CoV-2 entry and replication. © 2023 The Authors. Published by American Chemical Society.
Research Area(s)
- antisense oligonucleotides, apoptotic mimicry, extracellular vesicles, SARS-CoV-2, viral inhibition
Citation Format(s)
Red Blood Cell-Derived Extracellular Vesicles Display Endogenous Antiviral Effects and Enhance the Efficacy of Antiviral Oligonucleotide Therapy. / Jayasinghe, Migara K.; Gao, Chang; Yap, Gracemary et al.
In: ACS Nano, Vol. 17, No. 21, 14.11.2023, p. 21639-21661.
In: ACS Nano, Vol. 17, No. 21, 14.11.2023, p. 21639-21661.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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