Recovery of glucose and polyester from textile waste by enzymatic hydrolysis

In order to recover glucose and polyesterfrom textile waste, the enzymatic hydrolysis of alkali pretreated textile waste wasinvestigated. The influenceof key factors related to hydrolysisprocesswasevaluated, includingsubstrateloadingratio, temperature, cellulase dosage, as well as the supplementation ofβ-glucosidase. Results showed that increasing substrate loading from 1% to 7% had a negative effect on glucose recovery yield and thesignificant inhibitive effect wasobserved at over 3%substrate loading. Substrate loading at 3% was selected based on glucose yield and hydrolysis efficiency. The optimal temperature for enzymatic hydrolysis was 50 °Cand significant reduction was observed over 60 °C. More than 50IU/gβ-glucosidase did not contribute to the increase of glucose recovery yield. Therefore, theoptimum enzymatic hydrolysis condition was using 20 FPU/g cellulaseand 50 IU/g β-glucosidase at 50°C, based on the criterion forminimizingenzyme dosage and maximizing glucose recovery. The maximum glucose yield of 90%was achievedand the glucose production was 606mg/g textile waste after 96h hydrolysis.