Reconstructed chitosan with alkylamine for enhanced gene delivery by promoting endosomal escape

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Author(s)

  • Guojun Huang
  • Qi Chen
  • Wangteng Wu
  • Jianwei Wang
  • Hongzhen Bai
  • Guping Tang

Detail(s)

Original languageEnglish
Article number115339
Journal / PublicationCarbohydrate Polymers
Volume227
Online published19 Sep 2019
Publication statusPublished - 1 Jan 2020

Abstract

Poor buffering capacity of chitosan (CS) results in insufficient intracellular gene release which poses the major barrier in gene delivery. Herein, we reconstructed pristine CS with propylamine (PA), (diethylamino) propylamine (DEAPA), and N, N-dimethyl- dipropylenetriamine (DMAMAPA) to obtain a series of alkylamine-chitosan (AA-CS). The introduction of multiple amino groups with rational ratios functionally enhance the buffering capacity of AA-CS, among which DMAPAPA-CS showed buffering capacity of 1.58 times that of chitosan. The reconstructed AA-CS functionally enhance the ability of gene binding and endosomal escape. It was observed that the DMAPAPA-CS/pDNA complexes exhibit a notable gene delivery efficiency, which promotes the functionalization of loaded pDNA. Importantly, the in vivo delivery assay reveals that the deep penetration issue can be resolved using DMAPAPA-CS gene delivery vector. Finally, the DMAPAPA-CS is applied to deliver the therapeutic p53 gene in A549 bearing mice, showing efficient therapeutic potential for cancer.

Research Area(s)

  • Alkylamine, Buffering capacity, Chitosan, Endosomal escape, Gene delivery

Citation Format(s)

Reconstructed chitosan with alkylamine for enhanced gene delivery by promoting endosomal escape. / Huang, Guojun; Chen, Qi; Wu, Wangteng; Wang, Jianwei; Chu, Paul K.; Bai, Hongzhen; Tang, Guping.

In: Carbohydrate Polymers, Vol. 227, 115339, 01.01.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review