Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis

Hin Sum Man; Siying Wu; Tiffany Ng; Fu Kin Ho; Ting Hsuan Chen

doi:10.1109/NANOMED.2018.8641688

Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis

Hin Sum Man, Siying Wu, Tiffany Ng, Fu Kin Ho, Ting Hsuan Chen^*

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Inherent left-right (LR) asymmetry is commonly seen in tissue/organ microarchitecture. However, how to reconstitute such tissue morphogenesis remains elusive. Here we report a method to recapture the inherent LR asymmetry during myogenesis. C2C12 skeletal myoblasts were cultured and induced on micropatterned cell-adherent stripes with different stripe width ranging from 20 to 800 μm. Analysis of cell orientation revealed a significant LR bias on stripes with width of 50 and 80 μm before and after myogenic induction. Moreover, cells on such stripes also exhibited increased myotube formation, suggesting an optimized experimental condition for higher degree of myogenic differentiation with inherent LR-biased cell orientation. The result pave ways for reconstructing LR asymmetry in tissue morphogenesis in the future.

Original language	English
Title of host publication	IEEE-NANOMED 2018
Subtitle of host publication	The 12th IEEE International Conference on Nano/Molecular Medicine and Engineering
Publisher	IEEE
Pages	214-217
ISBN (Electronic)	978-1-5386-7579-3, 978-1-5386-7578-6
DOIs	https://doi.org/10.1109/NANOMED.2018.8641688
Publication status	Published - Dec 2018
Event	12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018) - Waikiki Beach, United States Duration: 2 Dec 2018 → 5 Dec 2018 http://www.ieee-nanomed.org/2018/

Publication series

Name	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Publisher	IEEE
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018)
Country/Territory	United States
City	Waikiki Beach
Period	2/12/18 → 5/12/18
Internet address	http://www.ieee-nanomed.org/2018/

Access Output

10.1109/NANOMED.2018.8641688

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

Man, H. S., Wu, S., Ng, T., Ho, F. K., & Chen, T. H. (2018). Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis. In IEEE-NANOMED 2018: The 12th IEEE International Conference on Nano/Molecular Medicine and Engineering (pp. 214-217). Article 8641688 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). IEEE. https://doi.org/10.1109/NANOMED.2018.8641688

@inproceedings{851935e8622a41c5816bc8861ecc918a,

title = "Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis",

abstract = "Inherent left-right (LR) asymmetry is commonly seen in tissue/organ microarchitecture. However, how to reconstitute such tissue morphogenesis remains elusive. Here we report a method to recapture the inherent LR asymmetry during myogenesis. C2C12 skeletal myoblasts were cultured and induced on micropatterned cell-adherent stripes with different stripe width ranging from 20 to 800 μm. Analysis of cell orientation revealed a significant LR bias on stripes with width of 50 and 80 μm before and after myogenic induction. Moreover, cells on such stripes also exhibited increased myotube formation, suggesting an optimized experimental condition for higher degree of myogenic differentiation with inherent LR-biased cell orientation. The result pave ways for reconstructing LR asymmetry in tissue morphogenesis in the future.",

author = "Man, {Hin Sum} and Siying Wu and Tiffany Ng and Ho, {Fu Kin} and Chen, {Ting Hsuan}",

year = "2018",

month = dec,

doi = "10.1109/NANOMED.2018.8641688",

language = "English",

series = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

publisher = "IEEE",

pages = "214--217",

booktitle = "IEEE-NANOMED 2018",

address = "United States",

note = "12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018) ; Conference date: 02-12-2018 Through 05-12-2018",

url = "http://www.ieee-nanomed.org/2018/",

}

Man, HS, Wu, S, Ng, T, Ho, FK & Chen, TH 2018, Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis. in IEEE-NANOMED 2018: The 12th IEEE International Conference on Nano/Molecular Medicine and Engineering., 8641688, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, IEEE, pp. 214-217, 12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018), Waikiki Beach, Hawaii, United States, 2/12/18. https://doi.org/10.1109/NANOMED.2018.8641688

Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis. / Man, Hin Sum; Wu, Siying; Ng, Tiffany et al.
IEEE-NANOMED 2018: The 12th IEEE International Conference on Nano/Molecular Medicine and Engineering. IEEE, 2018. p. 214-217 8641688 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis

AU - Man, Hin Sum

AU - Wu, Siying

AU - Ng, Tiffany

AU - Ho, Fu Kin

AU - Chen, Ting Hsuan

PY - 2018/12

Y1 - 2018/12

N2 - Inherent left-right (LR) asymmetry is commonly seen in tissue/organ microarchitecture. However, how to reconstitute such tissue morphogenesis remains elusive. Here we report a method to recapture the inherent LR asymmetry during myogenesis. C2C12 skeletal myoblasts were cultured and induced on micropatterned cell-adherent stripes with different stripe width ranging from 20 to 800 μm. Analysis of cell orientation revealed a significant LR bias on stripes with width of 50 and 80 μm before and after myogenic induction. Moreover, cells on such stripes also exhibited increased myotube formation, suggesting an optimized experimental condition for higher degree of myogenic differentiation with inherent LR-biased cell orientation. The result pave ways for reconstructing LR asymmetry in tissue morphogenesis in the future.

AB - Inherent left-right (LR) asymmetry is commonly seen in tissue/organ microarchitecture. However, how to reconstitute such tissue morphogenesis remains elusive. Here we report a method to recapture the inherent LR asymmetry during myogenesis. C2C12 skeletal myoblasts were cultured and induced on micropatterned cell-adherent stripes with different stripe width ranging from 20 to 800 μm. Analysis of cell orientation revealed a significant LR bias on stripes with width of 50 and 80 μm before and after myogenic induction. Moreover, cells on such stripes also exhibited increased myotube formation, suggesting an optimized experimental condition for higher degree of myogenic differentiation with inherent LR-biased cell orientation. The result pave ways for reconstructing LR asymmetry in tissue morphogenesis in the future.

UR - http://www.scopus.com/inward/record.url?scp=85063028968&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85063028968&origin=recordpage

U2 - 10.1109/NANOMED.2018.8641688

DO - 10.1109/NANOMED.2018.8641688

M3 - RGC 32 - Refereed conference paper (with host publication)

T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

SP - 214

EP - 217

BT - IEEE-NANOMED 2018

PB - IEEE

T2 - 12th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2018)

Y2 - 2 December 2018 through 5 December 2018

ER -

Reconstitution of Inherent Left-Right Asymmetry in Skeletal Myogenesis

Abstract

Publication series

Conference

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this