Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

1 Scopus Citations
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Author(s)

  • Yun He
  • Junhui Shi
  • Junjie Yao
  • Lei Li
  • Ruiying Zhang
  • Chih-Hsien Huang
  • Jun Zou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume10064
ISBN (Print)9781510605695
Publication statusPublished - Mar 2017

Publication series

Name
Volume10064
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2017
PlaceUnited States
CitySan Francisco
Period29 January - 1 February 2017

Abstract

Metastasis is responsible for as many as 90% of cancer-related deaths, and the deadliest skin cancer, melanoma, has a high propensity for metastasis. Since hematogenous spread of circulating tumor cells (CTCs) is cancer's main route of metastasis, detecting and destroying CTCs can impede metastasis and improve patients' prognoses. Extensive studies employing exogenous agents to detect tumor-specific biomarkers and guide therapeutics to CTCs have achieved promising results, but biosafety remains a critical concern. Taking another approach, physical detection and destruction of CTCs is a safer way to evaluate and reduce metastasis risks. Melanoma cells strongly express melanosomes, providing a striking absorption contrast with the blood background in the red to near-infrared spectrum. Exploiting this intrinsic optical absorption contrast of circulating melanoma cells, we coupled dual-wavelength photoacoustic flow cytography with a nanosecond-pulsed laser killing mechanism that specifically targets melanoma CTCs. We have successfully achieved in vivo label-free imaging of rare single CTCs and CTC clusters in mice. Further, the photoacoustic signal from a CTC immediately hardware-triggers a lethal pinpoint laser irradiation that lyses it on the spot in a thermally confined manner. Our technology can facilitate early inhibition of metastasis by clearing circulating tumor cells from vasculature.

Research Area(s)

  • Circulating tumor cell, Flow cytography, Laser therapy, Melanoma, Photoacoustic microscopy

Citation Format(s)

Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo. / He, Yun; Wang, Lidai; Shi, Junhui et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 10064 SPIE, 2017. 100641E.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review