Real-Time Imaging of Dynamic Cell Reprogramming with Nanosensors

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

9 Scopus Citations
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Author(s)

  • Christian Wiraja
  • David C. Yeo
  • Khek-Chian Tham
  • Sharon W. T. Chew
  • Xinhong Lim

Detail(s)

Original languageEnglish
Article number1703440
Journal / PublicationSmall
Volume14
Issue number17
Online published3 Apr 2018
Publication statusPublished - 26 Apr 2018
Externally publishedYes

Abstract

Cellular reprogramming, the process by which somatic cells regain pluripotency, is relevant in many disease modeling, therapeutic, and drug discovery applications. Molecular evaluation of reprogramming (e.g., polymerase chain reaction, immunostaining) is typically disruptive, and only provides snapshots of phenotypic traits. Gene reporter constructs facilitate live-cell evaluation but is labor intensive and may risk insertional mutagenesis during viral transfection. Herein, the utilization of a non-integrative nanosensor is demonstrated to visualize key reprogramming events in situ within live cells. Principally based on sustained intracellular release of encapsulated molecular probes, nanosensors successfully monitored mesenchymal-epithelial transition, pluripotency acquisition, and transdifferentiation events. Tracking the dynamic expression of four pivotal biomarkers (i.e., THY1, E-CADHERIN, OCT4, and GATA4 mRNA), nanosensor signal showed great agreement with polymerase chain reaction and gene reporter imaging (R2 > 0.9). Overall, such facile, versatile nanosensor enables real-time monitoring of low-frequency reprogramming events, thereby useful for high-throughput assessment, optimization, and biomarker-specific cell enrichment.

Research Area(s)

  • cell reprogramming, molecular beacons, nanosensors, non-integrative sensors, pluripotency, real-time monitoring

Citation Format(s)

Real-Time Imaging of Dynamic Cell Reprogramming with Nanosensors. / Wiraja, Christian; Yeo, David C.; Tham, Khek-Chian; Chew, Sharon W. T.; Lim, Xinhong; Xu, Chenjie.

In: Small, Vol. 14, No. 17, 1703440, 26.04.2018.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review