Real-time breath analysis by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

5 Scopus Citations
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Author(s)

  • Xue Li
  • Dan D. Huang
  • Rui Du
  • Zhi J. Zhang
  • Zheng X. Huang
  • Zhen Zhou

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article numbere56465
Journal / PublicationJournal of Visualized Experiments
Volume2018
Issue number133
Online published9 Mar 2018
Publication statusPublished - 2018

Abstract

Exhaled volatile organic compounds (VOCs) have aroused considerable interest, since they can serve as biomarkers for disease diagnosis and environmental exposure in a non-invasive manner. In this work, we present a protocol to characterize the exhaled VOCs in real time by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry (Sec-nanoESI-HRMS). The homemade Sec-nanoESI source was readily set up based on a commercial nanoESI source. Hundreds of peaks were observed in the background-subtracted mass spectra of exhaled breath, and the mass accuracy values are -4.0-13.5 ppm and -20.3-1.3 ppm in the positive and negative ion detection modes, respectively. The peaks were assigned with accurate elemental composition according to the accurate mass and isotopic pattern. Less than 30 s is used for one exhalation measurement, and it takes approximately 7 min for six replicated measurements.

Research Area(s)

  • Breath analysis, High resolution mass spectrometry, Real time, Secondary nanoelectrospray ionization, Volatile organic compounds

Citation Format(s)

Real-time breath analysis by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry. / Li, Xue; Huang, Dan D.; Du, Rui; Zhang, Zhi J.; Chan, Chak K.; Huang, Zheng X.; Zhou, Zhen.

In: Journal of Visualized Experiments, Vol. 2018, No. 133, e56465, 2018.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review