Real-Time measurement of single bacterium's refractive index using optofluidic immersion refractometry

P. Y. Liu; L. K. Chin; W. Ser; T. C. Ayi; P. H. Yap; T. Bourouina; Y. Leprince-Wang

doi:10.1016/j.proeng.2014.11.743

Real-Time measurement of single bacterium's refractive index using optofluidic immersion refractometry

P. Y. Liu
, L. K. Chin
, W. Ser
, T. C. Ayi
, P. H. Yap
, T. Bourouina
, Y. Leprince-Wang

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

60 Citations (Scopus)

15 Downloads (CityUHK Scholars)

Abstract

This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents.

Original language	English
Pages (from-to)	356-359
Journal	Procedia Engineering
Volume	87
DOIs	https://doi.org/10.1016/j.proeng.2014.11.743
Publication status	Published - 2014
Externally published	Yes
Event	28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy Duration: 7 Sept 2014 → 10 Sept 2014 http://www.sciencedirect.com/science/journal/18777058/87

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 6 Clean Water and Sanitation

Research Keywords

Immersion refractometer
Optofludics
Waterborne bacteria

Publisher's Copyright Statement

This full text is made available under CC-BY-NC-ND 3.0. https://creativecommons.org/licenses/by-nc-nd/3.0/

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96553254Final Published version, 598 KBLicence: CC BY-NC-ND

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title = "Real-Time measurement of single bacterium's refractive index using optofluidic immersion refractometry",

abstract = "This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents.",

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author = "Liu, \{P. Y.\} and Chin, \{L. K.\} and W. Ser and Ayi, \{T. C.\} and Yap, \{P. H.\} and T. Bourouina and Y. Leprince-Wang",

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language = "English",

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T1 - Real-Time measurement of single bacterium's refractive index using optofluidic immersion refractometry

AU - Liu, P. Y.

AU - Chin, L. K.

AU - Ser, W.

AU - Ayi, T. C.

AU - Yap, P. H.

AU - Bourouina, T.

AU - Leprince-Wang, Y.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014

Y1 - 2014

N2 - This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents.

AB - This paper presents a biophysical method to characterize single bacterium in water by using an on-chip optofluidic immersion refractometer. Water safety is a major factor in the well-being of people, but the presence of bacteria such as Escherichia coli (E. coli), bacillus subtilis, Shigella flexneri and vibrio cholera in drinking water can lead to infectious diseases such as typhoid fever. Hence, it is crucial to detect and identify bacteria to prevent bacterial outbreaks. In this paper, an optofluidic immersion refractometer is developed to measure three biophysical parameters, i.e. size, shape and refractive index. The refractive index of a single bacterium is measured in high sensitivity of 0.005 RIU. This system is an innovative method to allow on-site real-time detection of single bacterium in water. It significantly reduces the amount of detection time and do not require trained personnel or additional chemical and biological reagents.

KW - Immersion refractometer

KW - Optofludics

KW - Waterborne bacteria

UR - https://www.scopus.com/pages/publications/84923384751

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84923384751&origin=recordpage

U2 - 10.1016/j.proeng.2014.11.743

DO - 10.1016/j.proeng.2014.11.743

M3 - RGC 21 - Publication in refereed journal

SN - 1877-7058

VL - 87

SP - 356

EP - 359

JO - Procedia Engineering

JF - Procedia Engineering

T2 - 28th European Conference on Solid-State Transducers, EUROSENSORS 2014

Y2 - 7 September 2014 through 10 September 2014

ER -

Real-Time measurement of single bacterium's refractive index using optofluidic immersion refractometry

Abstract

Bibliographical note

UN SDGs

Research Keywords

Publisher's Copyright Statement

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