Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

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Original languageEnglish
Article numbere00208-18
Journal / PublicationMSYSTEMS
Volume4
Issue number1
Early online date22 Jan 2019
Publication statusPublished - Jan 2019

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Abstract

Microbial communities are composed of populations with vastly different abundances and levels of metabolic and replicative activity, ranging from actively metabolizing and dividing to dormant or nonviable. The 16S rRNA/rDNA ratio is an emerging tool for evaluating cell-level metabolic activity independent of abundance. In this study, we used five long-term enriched model anaerobic digestion (AD) communities to investigate community composition, diversity, structure, and in particular activity based on the rRNA/rDNA ratio. We cross-validated the 16S amplicon-based results using two alternative operational taxonomic unit (OTU) formation methods (conventional 97% sequence similarity and 100% sequence similar zero-radius OTUs by UNOISE3) and compared these to metagenome-derived population genomes and metatranscriptomes. Significant positive correlations were observed between microbial total activity and abundance with both the amplicon-and omic-based methods. All three methods revealed disproportionately high transcription/abundance ratios for some rare taxa but lower ratios for most abundant taxa for all the communities, which was further corroborated by the high replication rate (iRep) of most low-abundance population genomes.

Research Area(s)

  • anaerobic digestion, ZOTU, cellulose, population genome, rRNA/rDNA ratio, xylan, LONG-TERM ENRICHMENT, RIBOSOMAL-RNA, BACTERIAL COMMUNITIES, GROWTH-RATES, DIVERSITY, RESOLUTION, DYNAMICS, CONTRIBUTE, CELLULOSE, REFLECTS

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