Rapid screening of complex DNA samples by single-molecule amplification and sequencing

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

2 Scopus Citations
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Author(s)

  • Jiaqi Huang
  • Zongli Zheng
  • Anders F. Andersson
  • Lars Engstrand
  • Weimin Ye

Detail(s)

Original languageEnglish
Article numbere19723
Journal / PublicationPLoS ONE
Volume6
Issue number5
Publication statusPublished - 2011
Externally publishedYes

Abstract

Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. © 2011 Huang et al.

Citation Format(s)

Rapid screening of complex DNA samples by single-molecule amplification and sequencing. / Huang, Jiaqi; Zheng, Zongli; Andersson, Anders F.; Engstrand, Lars; Ye, Weimin.

In: PLoS ONE, Vol. 6, No. 5, e19723, 2011.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review