TY - JOUR
T1 - Rapid screening of complex DNA samples by single-molecule amplification and sequencing
AU - Huang, Jiaqi
AU - Zheng, Zongli
AU - Andersson, Anders F.
AU - Engstrand, Lars
AU - Ye, Weimin
PY - 2011
Y1 - 2011
N2 - Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. © 2011 Huang et al.
AB - Microbial cloning makes Sanger sequencing of complex DNA samples possible but is labor intensive. We present a simple, rapid and robust method that enables laboratories without special equipment to perform single-molecule amplicon sequencing, although in a low-throughput manner, from sub-picogram quantities of DNA. The method can also be used for quick quality control of next-generation sequencing libraries, as was demonstrated for a metagenomic sample. © 2011 Huang et al.
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-79956258795&origin=recordpage
U2 - 10.1371/journal.pone.0019723
DO - 10.1371/journal.pone.0019723
M3 - RGC 21 - Publication in refereed journal
C2 - 21625543
SN - 1932-6203
VL - 6
JO - PLOS ONE
JF - PLOS ONE
IS - 5
M1 - e19723
ER -