Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Wei-Ping Qian
  • Yue-Qiu Tan
  • Ying Chen
  • Ying Peng
  • Zhi Li
  • Guang-Xiu Lu
  • Marie C. Lin
  • Hsiang-Fu Kung
  • Li-Ka Shing

Detail(s)

Original languageEnglish
Pages (from-to)5385-5389
Journal / PublicationWorld Journal of Gastroenterology
Volume11
Issue number34
Publication statusPublished - 14 Sep 2005
Externally publishedYes

Abstract

Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. Methods: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. Results: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×107 and 1.67×107 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.14×105 and 3.02×105 copies of HBV DNA per mL in the semen. Conclusion: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. © 2005 The WJG Press and Elsevier Inc. All rights reserved.

Research Area(s)

  • Hepatitis B virus, Real-time polymerase chain reaction, Semen, Viral load

Citation Format(s)

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction. / Qian, Wei-Ping; Tan, Yue-Qiu; Chen, Ying; Peng, Ying; Li, Zhi; Lu, Guang-Xiu; Lin, Marie C.; Kung, Hsiang-Fu; He, Ming-Ling; Shing, Li-Ka.

In: World Journal of Gastroenterology, Vol. 11, No. 34, 14.09.2005, p. 5385-5389.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal