TY - GEN
T1 - Rapid measurement of AFP using AFP-specific aptamer on a microfluidic chip
AU - Huang, Chao-Jyun
AU - Lin, Hsin-I.
AU - Shiesh, Shu-Chu
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2012
Y1 - 2012
N2 - This study presents a new suction-type microfluidic system capable of rapid measurement of alpha-fetoprotein (AFP) by utilizing magnetic bead-based technologies. Two modules, including a suction-type incubator for the magnetic beads to capture AFP and acridinium ester (AE)-labeled anti-AFP antibodies and a microfluidic control module for sample transportation, were integrated into this microfluidic system. With the incorporation of AFP-specific aptamer-conjugated magnetic beads, the target AFP could be recognized and attracted onto the surface of the magnetic beads from the clinical sample. The entire process including two-step incubation and purification process could be automatically performed within 25 min, which is only about 20% of the time required when using a benchtop machine (for about 130 min). Besides, the total sample and reagent volume consumed is only 105 μL, which is significantly less than that required in a large system (410 μL). More importantly, experimental results showed that clinical human serum samples can also be accurately analyzed. The microfluidic system may be promising for point-of-care applications for AFP detection in the future. © 2012 IEEE.
AB - This study presents a new suction-type microfluidic system capable of rapid measurement of alpha-fetoprotein (AFP) by utilizing magnetic bead-based technologies. Two modules, including a suction-type incubator for the magnetic beads to capture AFP and acridinium ester (AE)-labeled anti-AFP antibodies and a microfluidic control module for sample transportation, were integrated into this microfluidic system. With the incorporation of AFP-specific aptamer-conjugated magnetic beads, the target AFP could be recognized and attracted onto the surface of the magnetic beads from the clinical sample. The entire process including two-step incubation and purification process could be automatically performed within 25 min, which is only about 20% of the time required when using a benchtop machine (for about 130 min). Besides, the total sample and reagent volume consumed is only 105 μL, which is significantly less than that required in a large system (410 μL). More importantly, experimental results showed that clinical human serum samples can also be accurately analyzed. The microfluidic system may be promising for point-of-care applications for AFP detection in the future. © 2012 IEEE.
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U2 - 10.1109/MEMSYS.2012.6170325
DO - 10.1109/MEMSYS.2012.6170325
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 874
EP - 877
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012)
Y2 - 29 January 2012 through 2 February 2012
ER -