Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)

Zhigang Cao; Li Yi; Xiangnan Liu; Jinyuan Shang; Yuening Cheng; Erkai Feng; Xingyu Liu; Yuping Fan; Xiaoliang Hu; Wenlong Cai; Feng Cong; Shipeng Cheng

doi:10.3389/fvets.2024.1413420

Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)

Zhigang Cao (Co-first Author)
, Li Yi (Co-first Author)
, Xiangnan Liu (Co-first Author)
, Jinyuan Shang
, Yuening Cheng
, Erkai Feng
, Xingyu Liu
, Yuping Fan
, Xiaoliang Hu
, Wenlong Cai^*
, Feng Cong^*
, Shipeng Cheng^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

7 Citations (Scopus)

58 Downloads (CityUHK Scholars)

Abstract

Canine distemper virus (CDV) is a highly contagious and potentially lethal virus that affects dogs and other members of the Canidae family, including wolves, foxes, and coyotes. Here, we present a fluorescent lateral flow immunoassay (FLFA) platform for the detection of CDV, which utilizes fluorescent microspheres - fusion protein monoclonal antibody (mAb)-labeled monoclonal antibody. The assay detected CDV within 5 min, with a detection limit threshold of 3 × 10² TCID₅₀/mL. Notably, the assay demonstrated no cross-reactivity with canine parvovirus, canine coronavirus, canine adenovirus, feline calicivirus, feline herpesvirus, or feline parvovirus. Field and clinical applicability of the assay was evaluated using 63 field samples, including 30 canine fecal samples, 18 swab samples, and 15 blood samples. The coincidence rate between the detection results of clinical samples obtained through FLFA and reverse transcription polymerase chain reaction (RT-PCR) was 96.83%. Thus, this assay offers a significant advancement for the rapid diagnosis of CDV at the point of care.

© 2024 Cao, Yi, Liu, Shang, Cheng, Feng, Liu, Fan, Hu, Cai, Cong and Cheng.

Original language	English
Article number	1413420
Journal	Frontiers in Veterinary Science
Volume	11
Online published	11 Jun 2024
DOIs	https://doi.org/10.3389/fvets.2024.1413420
Publication status	Published - 2024

Research Keywords

CDV
FLFA
fluorescent
microspheres
detection

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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title = "Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)",

abstract = "Canine distemper virus (CDV) is a highly contagious and potentially lethal virus that affects dogs and other members of the Canidae family, including wolves, foxes, and coyotes. Here, we present a fluorescent lateral flow immunoassay (FLFA) platform for the detection of CDV, which utilizes fluorescent microspheres - fusion protein monoclonal antibody (mAb)-labeled monoclonal antibody. The assay detected CDV within 5 min, with a detection limit threshold of 3 × 102 TCID50/mL. Notably, the assay demonstrated no cross-reactivity with canine parvovirus, canine coronavirus, canine adenovirus, feline calicivirus, feline herpesvirus, or feline parvovirus. Field and clinical applicability of the assay was evaluated using 63 field samples, including 30 canine fecal samples, 18 swab samples, and 15 blood samples. The coincidence rate between the detection results of clinical samples obtained through FLFA and reverse transcription polymerase chain reaction (RT-PCR) was 96.83\%. Thus, this assay offers a significant advancement for the rapid diagnosis of CDV at the point of care. {\textcopyright} 2024 Cao, Yi, Liu, Shang, Cheng, Feng, Liu, Fan, Hu, Cai, Cong and Cheng. ",

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author = "Zhigang Cao and Li Yi and Xiangnan Liu and Jinyuan Shang and Yuening Cheng and Erkai Feng and Xingyu Liu and Yuping Fan and Xiaoliang Hu and Wenlong Cai and Feng Cong and Shipeng Cheng",

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TY - JOUR

T1 - Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)

AU - Cao, Zhigang

AU - Yi, Li

AU - Liu, Xiangnan

AU - Shang, Jinyuan

AU - Cheng, Yuening

AU - Feng, Erkai

AU - Liu, Xingyu

AU - Fan, Yuping

AU - Hu, Xiaoliang

AU - Cai, Wenlong

AU - Cong, Feng

AU - Cheng, Shipeng

PY - 2024

Y1 - 2024

N2 - Canine distemper virus (CDV) is a highly contagious and potentially lethal virus that affects dogs and other members of the Canidae family, including wolves, foxes, and coyotes. Here, we present a fluorescent lateral flow immunoassay (FLFA) platform for the detection of CDV, which utilizes fluorescent microspheres - fusion protein monoclonal antibody (mAb)-labeled monoclonal antibody. The assay detected CDV within 5 min, with a detection limit threshold of 3 × 102 TCID50/mL. Notably, the assay demonstrated no cross-reactivity with canine parvovirus, canine coronavirus, canine adenovirus, feline calicivirus, feline herpesvirus, or feline parvovirus. Field and clinical applicability of the assay was evaluated using 63 field samples, including 30 canine fecal samples, 18 swab samples, and 15 blood samples. The coincidence rate between the detection results of clinical samples obtained through FLFA and reverse transcription polymerase chain reaction (RT-PCR) was 96.83%. Thus, this assay offers a significant advancement for the rapid diagnosis of CDV at the point of care. © 2024 Cao, Yi, Liu, Shang, Cheng, Feng, Liu, Fan, Hu, Cai, Cong and Cheng.

AB - Canine distemper virus (CDV) is a highly contagious and potentially lethal virus that affects dogs and other members of the Canidae family, including wolves, foxes, and coyotes. Here, we present a fluorescent lateral flow immunoassay (FLFA) platform for the detection of CDV, which utilizes fluorescent microspheres - fusion protein monoclonal antibody (mAb)-labeled monoclonal antibody. The assay detected CDV within 5 min, with a detection limit threshold of 3 × 102 TCID50/mL. Notably, the assay demonstrated no cross-reactivity with canine parvovirus, canine coronavirus, canine adenovirus, feline calicivirus, feline herpesvirus, or feline parvovirus. Field and clinical applicability of the assay was evaluated using 63 field samples, including 30 canine fecal samples, 18 swab samples, and 15 blood samples. The coincidence rate between the detection results of clinical samples obtained through FLFA and reverse transcription polymerase chain reaction (RT-PCR) was 96.83%. Thus, this assay offers a significant advancement for the rapid diagnosis of CDV at the point of care. © 2024 Cao, Yi, Liu, Shang, Cheng, Feng, Liu, Fan, Hu, Cai, Cong and Cheng.

KW - CDV

KW - FLFA

KW - fluorescent

KW - microspheres

KW - detection

U2 - 10.3389/fvets.2024.1413420

DO - 10.3389/fvets.2024.1413420

M3 - RGC 21 - Publication in refereed journal

C2 - 38919159

SN - 2297-1769

VL - 11

JO - Frontiers in Veterinary Science

JF - Frontiers in Veterinary Science

M1 - 1413420

ER -

Rapid lateral flow immunoassay for fluorescence detection of canine distemper virus (CDV)

Abstract

Research Keywords

Publisher's Copyright Statement

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