Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system

Yu-Hsin Liu; Kang-Yi Lien; Chih-Hung Wang; Jiunn-Jong Wu; Gwo-Bin Lee

doi:10.1109/NEMS.2011.6017302

Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system

Yu-Hsin Liu, Kang-Yi Lien, Chih-Hung Wang, Jiunn-Jong Wu, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future. © 2011 IEEE.

Original language	English
Title of host publication	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages	88-91
DOIs	https://doi.org/10.1109/NEMS.2011.6017302
Publication status	Published - 2011
Externally published	Yes
Event	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan, China Duration: 20 Feb 2011 → 23 Feb 2011

Publication series

Name	NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Conference

Conference	6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Place	Taiwan, China
City	Kaohsiung
Period	20/02/11 → 23/02/11

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Magnetic bead
MEMS
Microfluidics
MRSA
PCR

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10.1109/NEMS.2011.6017302

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Liu, Y.-H., Lien, K.-Y., Wang, C.-H., Wu, J.-J., & Lee, G.-B. (2011). Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (pp. 88-91). Article 6017302 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). https://doi.org/10.1109/NEMS.2011.6017302

Liu, Yu-Hsin ; Lien, Kang-Yi ; Wang, Chih-Hung et al. / Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system. NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. pp. 88-91 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

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title = "Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system",

abstract = "This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future. {\textcopyright} 2011 IEEE.",

keywords = "Magnetic bead, MEMS, Microfluidics, MRSA, PCR",

author = "Yu-Hsin Liu and Kang-Yi Lien and Chih-Hung Wang and Jiunn-Jong Wu and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",

year = "2011",

doi = "10.1109/NEMS.2011.6017302",

language = "English",

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Liu, Y-H, Lien, K-Y, Wang, C-H, Wu, J-J & Lee, G-B 2011, Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system. in NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems., 6017302, NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, pp. 88-91, 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011, Kaohsiung, Taiwan, China, 20/02/11. https://doi.org/10.1109/NEMS.2011.6017302

Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system. / Liu, Yu-Hsin; Lien, Kang-Yi; Wang, Chih-Hung et al.
NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 88-91 6017302 (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system

AU - Liu, Yu-Hsin

AU - Lien, Kang-Yi

AU - Wang, Chih-Hung

AU - Wu, Jiunn-Jong

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2011

Y1 - 2011

N2 - This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future. © 2011 IEEE.

AB - This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future. © 2011 IEEE.

KW - Magnetic bead

KW - MEMS

KW - Microfluidics

KW - MRSA

KW - PCR

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U2 - 10.1109/NEMS.2011.6017302

DO - 10.1109/NEMS.2011.6017302

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781612847757

T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

SP - 88

EP - 91

BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011

Y2 - 20 February 2011 through 23 February 2011

ER -

Liu YH, Lien KY, Wang CH, Wu JJ, Lee GB. Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system. In NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems. 2011. p. 88-91. 6017302. (NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems). doi: 10.1109/NEMS.2011.6017302

Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Access Output

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