Rapid formation of granules coupling n-DAMO and anammox microorganisms to remove nitrogen

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Chunshuang Liu
  • Tao Liu
  • Xiaoying Zheng
  • Jia Meng
  • Hui Chen
  • Shihu Hu
  • Jianhua Guo

Detail(s)

Original languageEnglish
Article number116963
Journal / PublicationWater Research
Volume194
Online published23 Feb 2021
Publication statusPublished - 15 Apr 2021
Externally publishedYes

Abstract

Granular sludge exhibits unique features, including rapid settling velocity, high loading rate and relative insensitivity against inhibitors, thus being a favorable platform for the cultivation of slow-growing and vulnerable microorganisms, such as anaerobic ammonium oxidation (anammox) bacteria and nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO) microorganisms. While anammox granules have been widely applied, little is known about how to speed up the granulation process of n-DAMO microorganisms, which grow even slower than anammox bacteria. In this study, we used mature anammox granules as biotic carriers to embed n-DAMO microorganisms, which obtained combined anammox + n-DAMO granules within 6 months. The results of whole-granule 16S rRNA gene amplicon sequencing showed the coexistence of anammox bacteria, n-DAMO bacteria and n-DAMO archaea. The microbial stratification along granule radius was further elucidated by cryosection-16S rRNA gene amplicon sequencing, showing the dominance of n-DAMO archaea and anammox bacteria at inner and outer layers, respectively. Moreover, the images of cryosection-fluorescence in situ hybridization (FISH) verified this stratification and also indicated a shift in microbial stratification. Specifically, n-DAMO bacteria and n-DAMO archaea attached to the anammox granule surface initially, which moved to the inner layer after 4-months operation. On the basis of combined anammox + n-DAMO granules, a practically useful nitrogen removal rate (1.0 kg N/m3/d) was obtained from sidestream wastewater, which provides new avenue to remove nitrogen from wastewater using methane as carbon source. © 2021 Elsevier Ltd.

Research Area(s)

  • Anaerobic methane oxidation, Anammox, Cryosection, Granule, Nitrite/nitrate-dependent anaerobic methane oxidation (n-DAMO), Sidestream nitrogen removal

Citation Format(s)

Rapid formation of granules coupling n-DAMO and anammox microorganisms to remove nitrogen. / Liu, Chunshuang; Liu, Tao; Zheng, Xiaoying et al.
In: Water Research, Vol. 194, 116963, 15.04.2021.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review