Rapid fluorescence-based measurement of toxicity in anaerobic digestion

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review

10 Scopus Citations
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Author(s)

  • Jian Lin Chen
  • Raphael Ortiz
  • Yeyuan Xiao
  • Terry W.J. Steele
  • David C. Stuckey

Detail(s)

Original languageEnglish
Pages (from-to)123-130
Journal / PublicationWater Research
Volume75
Publication statusPublished - 5 May 2015
Externally publishedYes

Abstract

A rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. By initially using a pure facultative anaerobic strain, Enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (PCP). The technique revealed significant metabolic changes in Enterococcus faecalis with a PCP spike ranging from 0.05 to 100 mg/L, and could detect PCP's toxicity to E. faecalis at a concentration of only 0.05 mg/L in 8 min. Furthermore, by extending this technique to a mixed anaerobic sludge, not only could the effect of 0.05-100 mg/L PCP be determined on anaerobic digestion metabolism within 10min, but also its rate of biogas production. These results suggest that a resazurin-based fluorescence measurement can potentially be incorporated into a microfluidic system to develop a biosensor for the real-time monitoring, control and early warning of toxicant/inhibitor loads in the influent to an anaerobic digestion system.

Research Area(s)

  • Anaerobic digestion, Biosensor, Pentachlorophenol, Resazurin, Toxicity

Citation Format(s)

Rapid fluorescence-based measurement of toxicity in anaerobic digestion. / Chen, Jian Lin; Ortiz, Raphael; Xiao, Yeyuan; Steele, Terry W.J.; Stuckey, David C.

In: Water Research, Vol. 75, 05.05.2015, p. 123-130.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalNot applicablepeer-review