Rapid detection of apoptosis in mammalian cells by using intact cell MALDI mass spectrometry

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Hongjuan Dong
  • Wei Shen
  • Myra Ting Wai Cheung
  • Yimin Liang
  • Hon Yeung Cheung
  • Günter Allmaier
  • Oscar Kin-Chung Au

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)5181-5189
Journal / PublicationAnalyst
Volume136
Issue number24
Publication statusPublished - 21 Dec 2011

Abstract

Detection of cell death has extensive applications and is of great commercial value. However, most current high-throughput cell viability assays cannot distinguish the two major forms of cell death: apoptosis and necrosis. Many apoptosis-specific detection methods exist but they are time consuming and labour intensive. In this work, we proposed a novel approach based on Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) for the specific detection of apoptosis in cultured mammalian cells. Buffer washed cells were directly mixed with a matrix solution and subsequently deposited onto the stainless steel target for MALDI analysis. The resulting mass spectrometric profiles were highly reproducible and can be used to reflect cell viability. Remarkably, the mass spectrometric profiles generated from apoptotic cells were distinct from those from either normal or necrotic cells. The apoptosis-specific features of the mass spectra were proportional to the percentage of apoptotic cells in the culture, but are independent of the drugs used to stimulate apoptosis. This is the first report on the utilization of intact cell MALDI mass spectrometry in detecting mammalian cell apoptosis, and can be used as a basis for the development of a reliable, fast, label-free and high-throughput method for detecting apoptotic cell death. © The Royal Society of Chemistry.

Citation Format(s)

Rapid detection of apoptosis in mammalian cells by using intact cell MALDI mass spectrometry. / Dong, Hongjuan; Shen, Wei; Cheung, Myra Ting Wai; Liang, Yimin; Cheung, Hon Yeung; Allmaier, Günter; Kin-Chung Au, Oscar; Lam, Yun Wah.

In: Analyst, Vol. 136, No. 24, 21.12.2011, p. 5181-5189.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal