Rapid Detection of Antibody in Biological Fluids on a Bioarray Chip

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

2 Scopus Citations
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Author(s)

  • Yue Sun
  • Mahsa Gharibi Marzancola
  • Jonathan Lee
  • Naveed Gulzar
  • Jamie K. Scott
  • Shumei Wang

Detail(s)

Original languageEnglish
Pages (from-to)839-851
Journal / PublicationAnalytical Letters
Volume52
Issue number5
Publication statusPublished - 24 Mar 2019
Externally publishedYes

Abstract

A bioarray chip was developed for the rapid and sensitive detection of the antibody (Ab) in nanoliter volumes of biological fluids. The chip was used to detect the anti-hemagglutinin (anti-HA) Ab from mouse ascites fluids. On the bioarray chip, lanes of antigens (i.e., a 13-amino acid peptide, HA, that is bound by anti-HA Ab) were printed in one dimension on the chip surface. The antigens probed the protein samples (i.e., mouse anti-HA) obtained from biological fluids flowing in channels arranged in the perpendicular dimension. To determine if the detection sensitivity was increased by using different fluorescent labeling methods, two types of labeling reagents were compared, namely AlexaFluor 647-labeled goat anti-mouse Ab (AxIgG), and biotin-labeled anti-mouse Ab (BioIgG) followed by cyanine 5-tagged streptavidin (SA-Cy5). In addition, the assay procedure was shortened by omitting the reduction and blocking steps, while maintaining the sensitivity. In conclusion, a bioarray chip was adopted to detect as low as 10 pM mouse monoclonal antibody (anti-HA) in 500 nL of biological fluids (i.e., corresponding to 5 attomoles of antibody). Our results demonstrate that on-chip detection using this bioarray format is more sensitive and less time-consuming than traditional analyses.

Research Area(s)

  • Anti-hemagglutinin, bioarray chip, monoclonal antibody, surface functionalization

Bibliographic Note

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Citation Format(s)

Rapid Detection of Antibody in Biological Fluids on a Bioarray Chip. / Sun, Yue; Gharibi Marzancola, Mahsa; Lee, Jonathan; Li, Paul C. H.; Gulzar, Naveed; Scott, Jamie K.; Wang, Shumei.

In: Analytical Letters, Vol. 52, No. 5, 24.03.2019, p. 839-851.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review