TY - GEN
T1 - Rapid bacteriophage detection via host cell amplication in a droplet-based optofluidic system
AU - Yu, J. Q.
AU - Huang, W.
AU - Chin, L. K.
AU - Liu, A. Q.
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2012
Y1 - 2012
N2 - This paper presents a real-time and label-free bacteriophage detection technique with high sensitivity and low cost by using a droplet-based optofluidic system. The bacteriophages and host cells (E. coli) mixture are encapsulated in microdroplets during their formation in mineral oil. The scattering patterns of the bacteriophages and the host cells containing droplets are captured by an optical sensor and analyzed by a signal processing system. The scattering patterns of microdroplets with different host cell concentrations are measured. The optofluidic system is developed to effectively detect and quantify bacteriophages in real-time with a detection resolution of 10 3. A higher resolution can be realized by using a data processing system to analyze the scattering pattern, which will be done in our future work.
AB - This paper presents a real-time and label-free bacteriophage detection technique with high sensitivity and low cost by using a droplet-based optofluidic system. The bacteriophages and host cells (E. coli) mixture are encapsulated in microdroplets during their formation in mineral oil. The scattering patterns of the bacteriophages and the host cells containing droplets are captured by an optical sensor and analyzed by a signal processing system. The scattering patterns of microdroplets with different host cell concentrations are measured. The optofluidic system is developed to effectively detect and quantify bacteriophages in real-time with a detection resolution of 10 3. A higher resolution can be realized by using a data processing system to analyze the scattering pattern, which will be done in our future work.
KW - Bacteriophage
KW - Optofluidics
KW - Scattering pattern
UR - https://www.scopus.com/pages/publications/84901780789
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84901780789&origin=recordpage
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1768
EP - 1770
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -