Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue

Hyunwoo Yuk; Jingjing Wu; Tiffany L. Sarrafian; Xinyu Mao; Claudia E. Varela; Ellen T. Roche; Leigh G. Griffiths; Christoph S. Nabzdyk; Xuanhe Zhao

doi:10.1038/s41551-021-00769-y

Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue

Hyunwoo Yuk^* (Co-first Author)
, Jingjing Wu (Co-first Author)
, Tiffany L. Sarrafian
, Xinyu Mao
, Claudia E. Varela
, Ellen T. Roche
, Leigh G. Griffiths
, Christoph S. Nabzdyk^*
, Xuanhe Zhao^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

334 Citations (Scopus)

Abstract

Tissue adhesives do not normally perform well on tissues that are covered with blood or other bodily fluids. Here we report the design, adhesion mechanism and performance of a paste that haemostatically seals tissues in less than 15 s, independently of the blood-coagulation rate. With a design inspired by barnacle glue (which strongly adheres to wet and contaminated surfaces owing to adhesive proteins embedded in a lipid-rich matrix), the paste consists of a blood-repelling hydrophobic oil matrix containing embedded microparticles that covalently crosslink with tissue surfaces on the application of gentle pressure. It slowly resorbs over weeks, sustains large pressures (approximately 350 mm Hg of burst pressure in a sealed porcine aorta), makes tough (interfacial toughness of 150–300 J m⁻²) and strong (shear and tensile strengths of, respectively, 40–70 kPa and 30–50 kPa) interfaces with blood-covered tissues, and outperforms commercial haemostatic agents in the sealing of bleeding porcine aortas ex vivo and of bleeding heart and liver tissues in live rats and pigs. The paste may aid the treatment of severe bleeding, even in individuals with coagulopathies. © The Author(s), under exclusive licence to Springer Nature Limited 2021.

Original language	English
Pages (from-to)	1131-1142
Number of pages	12
Journal	Nature Biomedical Engineering
Volume	5
Issue number	10
Online published	9 Aug 2021
DOIs	https://doi.org/10.1038/s41551-021-00769-y
Publication status	Published - Oct 2021
Externally published	Yes

Funding

We thank the Koch Institute Swanson Biotechnology Center for technical support, specifically K. Cormier and the Histology Core for the histological processing, and R. Bronson at Harvard Medical School for the histological evaluations. This work is supported by the MIT Deshpande Center (H.Y., C.S.N., X.Z.), National Institutes of Health (1-R01-HL153857-01, X.Z.), National Science Foundation (EFMA-1935291, X.Z.), the US Army Research Office through the Institute for Soldier Nanotechnologies at MIT (W911NF-13-D-0001) and the ZOLL Foundation (C.S.N.). H.Y. acknowledges financial support from Samsung Scholarship.

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title = "Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue",

abstract = "Tissue adhesives do not normally perform well on tissues that are covered with blood or other bodily fluids. Here we report the design, adhesion mechanism and performance of a paste that haemostatically seals tissues in less than 15 s, independently of the blood-coagulation rate. With a design inspired by barnacle glue (which strongly adheres to wet and contaminated surfaces owing to adhesive proteins embedded in a lipid-rich matrix), the paste consists of a blood-repelling hydrophobic oil matrix containing embedded microparticles that covalently crosslink with tissue surfaces on the application of gentle pressure. It slowly resorbs over weeks, sustains large pressures (approximately 350 mm Hg of burst pressure in a sealed porcine aorta), makes tough (interfacial toughness of 150–300 J m−2) and strong (shear and tensile strengths of, respectively, 40–70 kPa and 30–50 kPa) interfaces with blood-covered tissues, and outperforms commercial haemostatic agents in the sealing of bleeding porcine aortas ex vivo and of bleeding heart and liver tissues in live rats and pigs. The paste may aid the treatment of severe bleeding, even in individuals with coagulopathies. {\textcopyright} The Author(s), under exclusive licence to Springer Nature Limited 2021.",

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Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue. / Yuk, Hyunwoo (Co-first Author); Wu, Jingjing (Co-first Author); Sarrafian, Tiffany L. et al.
In: Nature Biomedical Engineering, Vol. 5, No. 10, 10.2021, p. 1131-1142.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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AU - Roche, Ellen T.

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Rapid and coagulation-independent haemostatic sealing by a paste inspired by barnacle glue

Abstract

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