A novel gelatin-based micro-cavitary hydrogel for potential application in delivery of anchorage dependent cells : A study with vasculogenesis model

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)334-342
Journal / PublicationColloids and Surfaces B: Biointerfaces
Volume146
Online published18 Jun 2016
Publication statusPublished - 1 Oct 2016
Externally publishedYes

Abstract

Hydrogels have been widely regarded as promising tissue engineering scaffolds and cell delivery vehicles, however, their inherent submicron- or nano-scale polymer networks severely inhibit the settlement of anchorage dependent cells (ADCs). Here, using endothelial progenitor outgrowth cells (EPOCs) as the typical ADCs, a gelatin-based micro-cavitary gel (namely Gel-MCG) is developed with gelatin-methacrylate and gelatin microspheres as precursor and porogens, respectively, to promote cellular focal adhesion and functions. The introduction of micro-cavitary structures within the Gel-MCG improves its physical properties as well as creates numerous gel-microcavity interfaces within gel-based matrices. Compared with conventional gelatin gel (Gel-G) scaffold, the Gel-MCG provides more suitable microenvironments for EPOCs' attachment, spreading, and proliferation, and then which leads to enhanced endothelial differentiation and vascularization as demonstrated by higher expressions of endothelial markers. The Gel-MCG system shows great potential as vehicle for the delivery of ADCs in tissue engineering. © 2016 Elsevier B.V.

Research Area(s)

  • Gelatin, Hydrogel, Porous structure, Tissue engineering, Vascularization

Citation Format(s)

A novel gelatin-based micro-cavitary hydrogel for potential application in delivery of anchorage dependent cells : A study with vasculogenesis model. / Leong, Wenyan; Fan, Changjiang; Wang, Dong-An.

In: Colloids and Surfaces B: Biointerfaces, Vol. 146, 01.10.2016, p. 334-342.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review