Harnessing J-aggregation for dual-color cellular imaging with chromenoquinoline-benzimidazole dyes
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Pages (from-to) | 7173-7179 |
Journal / Publication | Organic and Biomolecular Chemistry |
Volume | 22 |
Issue number | 35 |
Online published | 19 Aug 2024 |
Publication status | Published - 21 Sept 2024 |
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Abstract
Fluorescence imaging has revolutionized the visualization of cellular structures and biomolecules due to its non-invasive nature and high sensitivity. Chromenoquinoline (CQ)-based dyes offer promising optical properties, yet their widespread application is hindered by aggregation-caused quenching (ACQ). In contrast, J-aggregates, characterized by distinctive photophysical properties, present a solution to ACQ. Here, we introduce a novel platform employing chromenoquinoline-benzimidazole (CQ-BI) dyes, capable of forming J-aggregates, for dual-color cellular imaging. The incorporation of a methyl group into the benzimidazole moiety enhances J-aggregate formation, leading to robust emission in both dilute solutions and aggregated states. Our study demonstrates that methyl moiety-modified CQ-BI derivatives enable simultaneous imaging of mitochondria and lipid droplets in living cells. This work underscores the potential of CQ-BI dyes for dual-channel fluorescence imaging, leveraging the unique properties of J-aggregation. © 2024 The Royal Society of Chemistry.
Citation Format(s)
Harnessing J-aggregation for dual-color cellular imaging with chromenoquinoline-benzimidazole dyes. / Shangguan, Huimin; Teng, Zixuan; Ren, Xiaojie et al.
In: Organic and Biomolecular Chemistry, Vol. 22, No. 35, 21.09.2024, p. 7173-7179.
In: Organic and Biomolecular Chemistry, Vol. 22, No. 35, 21.09.2024, p. 7173-7179.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review