Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia

Kang-Yi Lien; Chien-Ju Liu; Pao-Lin Kuo; Gwo-Bin Lee

doi:10.1109/MEMSYS.2009.4805388

Author(s)

Kang-Yi Lien
Chien-Ju Liu
Pao-Lin Kuo
Gwo-Bin Lee

Detail(s)

Original language	English
Article number	4805388
Pages (from-to)	340-343
Journal / Publication	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Publication status	Published - 2009
Externally published	Yes

Conference

Title	22nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2009
Place	Italy
City	Sorrento
Period	25 - 29 January 2009

Link(s)

DOI	DOI https://doi.org/10.1109/MEMSYS.2009.4805388 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-65949110604&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(a603e151-e174-4b6c-baa4-9ab169a4b71a).html

Abstract

This study presents a new magnetic bead-based microfluidic platform for genomic DNA (gDNA) extraction and rapid detection of genetic deletion from saliva samples by using micro-electro-mechanical-systems (MEMS)-based technologies. With the incorporation of several microfluidic devices, a charge-switchable, magnetic bead was used to extract gDNA released from the buccal cells in the saliva samples and collected by a built-in microcoils array. A new normally-closed micropump for liquid delivery and a new self-compensated micro temperature control module for on-chip PCR were all integrated into a single chip. Successful demonstration for detection of the patients with genetic deletion has been achieved by using the proposed microfluidic platform. Consequently, the integrated miniature saliva-based microfluidic system can provide a promising platform for rapid DNA extraction and fast detection of genetic diseases in a shorter period of time automatically. ©2009 IEEE.

Bibliographic Note

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Citation Format(s)

[ RIS ] [ BibTeX ]

Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia. / Lien, Kang-Yi; Liu, Chien-Ju; Kuo, Pao-Lin et al.
In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 2009, p. 340-343.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Lien, K-Y, Liu, C-J, Kuo, P-L & Lee, G-B 2009, 'Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia', Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 340-343. https://doi.org/10.1109/MEMSYS.2009.4805388

Lien, K.-Y., Liu, C.-J., Kuo, P.-L., & Lee, G.-B. (2009). Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 340-343. Article 4805388. https://doi.org/10.1109/MEMSYS.2009.4805388

Lien KY, Liu CJ, Kuo PL, Lee GB. Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2009;340-343. 4805388. doi: 10.1109/MEMSYS.2009.4805388

Lien, Kang-Yi ; Liu, Chien-Ju ; Kuo, Pao-Lin et al. / Bead-Based microfluicid platform integrated with optical detection devices for rapid detection of genetic deletion from salvia. In: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2009 ; pp. 340-343.