Free chlorine loss during spray of membrane-less acidic electrolyzed water (MLAEW) and its antimicrobial effect on airborne bacteria from poultry house

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

6 Scopus Citations
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Author(s)

  • Yang Zhao
  • Hongwei Xin
  • Deling Zhao
  • Weichao Zheng
  • Wei Tian
  • He Ma
  • Hui Hu
  • Tong Wang
  • Michelle Soupir
  • Wei Wang

Detail(s)

Original languageEnglish
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013 (ASABE 2013)
PublisherAmerican Society of Agricultural and Biological Engineers
Pages3427-3437
Volume4
ISBN (Print)9781627486651
Publication statusPublished - Jul 2013
Externally publishedYes

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting (ASABE)
PublisherAmerican Society of Agricultural and Biological Engineers

Conference

TitleAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013 (ASABE 2013)
PlaceUnited States
CityKansas City
Period21 - 24 July 2013

Abstract

Spray-application of membrane-less acidic electrolyzed water (MLAEW) is a novel technique for disinfection in livestock houses. This study investigated the loss of free chlorine (FC, the major germicidal component in MLAEW) over distance during spray, as affected by air temperature and initial FC concentration. The antimicrobial effect of MLAEW on airborne bacteria from an aviary laying-hen house was examined. MLAEW was prepared with two FC concentrations (app. 15 and 60 mg L-1), and was sprayed at three air temperatures (18, 25, 32°C). The original MLAEW solution and MLAEW aerosols collected at 0, 25, and 50 cm from the spray nozzle were analyzed for FC concentrations. Bacteria were immersed into these MLAEW samples and numerated for viable count after 0.5-, 2-, and 5-min treatments. MLAEW aerosols collected at 0 cm lost 11.7 - 13.2% FC as compared to the original MLAEW solution. This initial loss was affected neither by the initial FC concentration (P = 0.13) nor by air temperature (P = 0.57). The rate of FC loss during travelling was 0.79 - 0.87% per centimeter of aerosol travel distance (% cm-1) at 18°C, 1.08 - 1.15% cm-1 at 25°C, and 1.35 - 1.49% cm-1 at 32°C. This travelling loss was affected by air temperature (P = 0.02), but not by initial FC concentration (P = 0.38). Bacteria were completely inactivated in 0.5 min when treated with MLAEW samples with FC > 16.8 mg L-1 , in 2 min when FC > 13.8 mg L-1 , and in 5 min when FC > 7.2 mg L-1. Airborne bacteria from aviary hen house can be effectively inactivated by MLAEW with adequate FC concentration and contact time. During spray, antimicrobial efficacy of MLAEW aerosols decreased over distance due to FC loss which exacerbates at higher air temperature.

Research Area(s)

  • Electrolyzed water, inactivation, airborne microorganisms, livestock, spraying

Citation Format(s)

Free chlorine loss during spray of membrane-less acidic electrolyzed water (MLAEW) and its antimicrobial effect on airborne bacteria from poultry house. / Zhao, Yang; Xin, Hongwei; Zhao, Deling et al.
American Society of Agricultural and Biological Engineers Annual International Meeting 2013 (ASABE 2013). Vol. 4 American Society of Agricultural and Biological Engineers, 2013. p. 3427-3437 131618610 (American Society of Agricultural and Biological Engineers Annual International Meeting (ASABE)).

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review